Grifola Frondosa Fermentation Of Traditional Chinese Medicine For The Matrix

Grifola frondosa is a kind of fungi both edible and medical,of which the extro-polysaccharide has the functions of anti-tumor and enhance the body immunity. this study was mainly focused on the extro-polysaccharide yield of Grifola frondosa by adding six GuiZhou Local materials,such as Buckwheat,Rhizoma Gastrodiae etc.1,Our study proved that Grifola frondosa could transform the active ingredients in Chinese herb and promote their activities,in addition,the ingredients in Chinese herb could also promote the yield of Grifola frondosa extro-polysaccharide.2,The test results of Grifola frondosa extro-polysaccharide extracting process flow was:take 100mL zymotic fluid,centrifuge in 4000r/min for 20min.then adding ethanol to 30%,keep in 4℃for one night.Centrifuge in 4000r/min for 1min. adding ethanol to the fluid to 75%,keep in 4℃for one night.Centrifuge in 4000r/min for 1min.parch the deposit.3,The Grifola frondosa submerged culture medium composition was:glucose 5g/L, peptone 5g/L,KH₂PO₄ 4g/L,MgSO₄ 1g/L;and the culture condition was:natural initial pH,seed age 5d,inoculums concentration 10%,250mL triangular flask containing 100mL,and culture under 150r/min and 25℃.4,The best adding quantities of Buckwheat,Rhizoma Gastrodiae,Dioscorea opposite Thunb ethanol extract was 6g/L,6g/L,8g/L.and the Grifola frondosa extro-polysaccharide yield was 3.28g/L,2.31g/L,3.42g/L.5,Take two of Buckwheat,Rhizoma Gastrodiae,Dioscorea opposite Thunb ethanol extract in to consideration,the mix of Buckwhea and Rhizoma Gastrodiae was best,the scale was 1:2,and the yield of extro-polysaccharide could reach 3.96g/L.6,This study optimized the data by responding surface method,the result showed that the yield of Grifola frondosa extropolysaccharide was 4.6052g/L when the ethanol extract quantities of Buckwhea and Rhizoma Gastrodiae was 3.0801g/L, 9.4019g/L.