Studies On The Extraction, Purification And Hypoglycemic Activity Of Extracellular Polysaccharide From Grifola Frondosa

This paper systematically studied on exo-polysaccharide from Grifola frondosa (GFP). Its fundamental physicochemical properties such as sugar content, specific rotation, ultraviolet scanning, infrared scanning and gas chromatography were investigated in this paper. Component separation was conducted by Sephadex G-200 to the GFP. A fraction (named GFP-1) was obtained after the inhibition of each segment onα-glucosidase. GFP-1 was detected inhibitory activity toα- glucosidase with the IC50 value under standard system. The inhibition type was non-competitive inhibition. Alloxan-induced diabetic mice were used to test the hypoglycemic activity of GFP. The synergistic effect between hypoglycemic activity and immunoregulation was confirmed in this paper.Extract exo-polysaccharide by alcohol sedimentation after strain activating and fermentation culture. The physicochemical properties studies on GFP confirmed it might be a new hetero-polysaccharide. The detection showed that the segment of GFP-1 had a high affinity and inhibitory activity withα-glucosidase. It had a research perspective in the treatment of diabetes. Through further verification in vivo test, GFP was confirmed not only has hypoglycemic effect but also can effectively improve glucose tolerance in mice, lower glucose area under the curve (AUC), and has no side effect. GFP also played a protective role on thymus, spleen and other immune organ. When the blood glucose in the mice was down regulated to the normal range, 10 days after the drug withdraw, it is found that GFP mice still maintained the blood glucose at a relatively low level, but the blood glucose level of positive treated mice was gradually increased. It indicates that GFP could control the glucose level via the regulation of body metabolism without polysaccharide dependence, while GFP also benefits kidney, which is the most common harmed organ by diabetes.