Of Cd, Hg And Tcb Stress On The Rice Proteome And Induction Of Protein Function Analysis

In the experiment, the responses of rice (Oryza sativa L., cv. ShanYou 63 and AiZaiZhan) to chenmical pollutant stress (Cd, Hg and TCB) were investigated by proteomic analysis. Conclution presented in this thesis can be summarized as follow:1.The effect of Cd, Hg and TCB on growth of rice seedlingsCd, Hg and TCB could decrease the relative water content of rice seedlings, which indicated that Cd, Hg and TCB could prevent rice roots absorbing water.Cd, Hg and TCB could increase the MDA content in rice leaves and roots significantly, which indicated that Cd, Hg and TCB could cause membrance lipid-peroxidation, hence, the structure and physiological functions of membrance were damaed.Cd, Hg and TCB could influence the protein metabolism. The protein content in leaves and roots decreased with the concentration of Cd, Hg and TCB increasing. While, the protein content have a little increase when the concentration of Cd and TCB was low.2.The effect of Cd, Hg and TCB on proteome in rice leaves and rootsCd, Hg and TCB stress had significant effect on global proteome in rice roots and leaves. The expression of considerable amount of proteins were either inhibited or induced by Cd, Hg and TCB stress.The analysis of the category and function of stress inducible proteins showed that different group of proteins were induced in response to TCB and Cd stress. They are detoxification enzymes (including esterase and aldo/keto reductase family protein in the roots, glutathions S-transferase in both roots and leaves, and peroxidase in the leaves), cell wall compound metabolism related enzymes (including UDP-glucose protein transglucosylase and GDP-mannose 3,5-epimerase 1 in the roots,α-L-arabinofuranosidase in the leaves),phytohormone metabolism and regulation related enzymes or protein (including aci-reductone dioxygenase 4 andβ-glucosidase in the roots, pathogenesis-related protein family 10 proteins in the roots or leaves), primary and secondary metabolism regulative enzymes (including ubiquitin protein in the leaves, translational elongation factor Tu, cytosolic orthophosphate dikinase, alanine aminotransferases and isoflavone reductase in the roots, different triosephosphate isomerases in the roots and leaves).Compared the TCB and Cd stress inducible proteins between two cultivars,β-glucosidase and PR10 proteins were particularly induced in Aizaizhan roots, and glutathione S-transferase and aci-reductone dioxygenase 4 were particularly induced in Shanyou63 roots. This might be one of the important mechanisms for Shanyou63 having higher tolerance to TCB and Cd stress than Aizaizhan.