The Five Viewing The Gametophyte Of Mosses And Tissue Culture

By means of in vitro gametophyte culture and spore culture, regeneration systems were established for five bryophyte species, e.g. Plagiomnium cuspidatum (Hedw.) T. Kop., Bartramia pomiformis Hedw., Ceratodon purpureus (Hedw.) Brid., Taxiphyllum taxirameum (Mitt.) Fleisch. and Polytrichum juniperinum Hedw.. Effects of phytohormones on multip- lication of P.cuspdatum were also dealt with in this paper. The results were listed as follows:1 Multiplication system of P. cuspidatum was estabilished from stem segment. First, sterilized the surface of shoot tips of P. cuspidatum with 1.0% concentration of sodium hypochlorite(freshly) and a drop of suds for 4min, then washed them with axenic distilled water several times, each lasting for 30 seconds. Sip up the surface with sterile filter paper before they were cut segments of 1cm length, and after inoculating, regular subculturing was necessary every 60 days. 45g/L sucrose concentration had an optimal effect on P.cuspdatum's multiplication. Pertinent phytohormone concentration, 0.02mg/L BA , 0.2mg/L 2,4-D, 0.02mg/L NAA, 1mg/L GA3, respectively, received optimal multiplication effects for this species. TDZ did not, even negatively, promate its growth.2 Tissue culture system of B. pomiformis was established. Intact capsules of B. pomiformis were picked (with 1cm seta) to be sterilized with sodium hypochlotite (always freshly prepared), lasting 6 min. After washed by distilled water several times, filter paper were used to sip up water on capsules surface; The modified Knop medium was used for medium of culturing protonema; The medium inducing callus was MS+ BA0.5mg/L+2,4-D0.1mg/L+ 4%sucrose+ 0.8%agar(pH5.8), subculturing per 30 days; The best medium of generation of gametophyte was Knop if explant was callus, while if explant was axenic little gametophyte, the best one was MS which could induce more little gametophyte.3 In vitro culture system of C .purpureus was established primarily. Sporephyte of C .purpureus was disinfected by 1.0% sodium hypochlorite, lasting 6 min, and then washed it. The better medium of callus was Knop addition of BA1.0mg/L; MS medium, which fit to induce regeneration from gametophyte, was also used for redifferentiating of callus.4 1.0% sodium hypochlorite containing drops of suds was used to sterilizing T. taxirameum 8min, the effects as for 1%HgCl2 2min and 1.0%sodium hypochlorite 6min on sterilizing P. juniperinum.5 Germination of spores of B. pomiformis, C. purpureus, T. taxirameum and P. juniperinum were all attributed to ectoangial type, spore germinating of B. pomiformis was Bryum type, P. juniperinum was Funaria type, and Spore germinating of T. taxirameum was Schistostega type. The multiplication systems established for five bryophyte species provided useful data for other mosses. Feather, discussion on approaches of gametophyte developing was given finally.