| The essay covers the new characteristics which have appeared in animal infection and are popular in recent years. Because the virus disease and immunity retarting disease are prevalent at a large scale, the immunity of pig organs has barriar and decline to resist disease, leading to the infection of mutil-pathogen complication and secondary disease. As the cases are complex, it is hard for people use antibiotic substance to control. Owing to adding a lot of ingredient of antibiotic substance to stock feed and drinking water has caused the animal organs to fail to resist disease and the serous problem of medicine remaining in animal products. When the tolerated strain appears constantly, it will do harm to the human health potentially. Therefore antibiotic substance used to animals will be restricted more and more. The stock immunostimulant may reduce the use of antibiotic substance and will play a positive part in dealing with the stock disease prevention, the safety of stock products and environmental pollution.The essay states the classifications of stock immunostimulant, common immunostimulant characteristics, reasearch development and some characteristics and the condition of immunostimulant applied to feed. The essay puts forward an idea that mannatide as a kind of feed immunostimulant can be applied to cultivating industry .The essay chooses Duroc-Landrace-Yorkshire crossing piglets as tested animals and studies, mannatide impacting on piglet growth, digestion as well as immunity. The results of the experiment are as follow:1. As to the growthTo compare contrasted group, to add 0.10% and 0.15% mannatide to increase survival rate and upgrade daily gain 8.34% (P < 0.05) and 8.34% (P < 0.05), 7.69% (P < 0.05 ) and 9.32% (P < 0.01) ,to lower 6.87% (P < 0.05) and 6.87% (P < 0.05), 32.65% (P < 0.01) and 33.92% (P < 0.01) in terms of feed-weight rate and diarrhoea. To add 0.05% mannatide can make diarrhoea rate lower 16.35% (P < 0.05) while raising survival rate and daily gain and lower feed-weight rate.To compare different experimental groups with the contrasted group , the daily feed intake has no obvious impact (P>0.05) .2. As to the colon and digestion functionsTo compare contrasted group, to add 0.10% and 0.15% mannatide to daily gain can make bifidobacteria and lactobacillus of colons increase 299.49% (P < 0.01) and 314.81% (P < 0.01), 97.40% (P < 0.05) and 141.67% (P < 0.05), and make the PH of objects inside the colons lower 0.50 (P < 0.05 ) and 0.90 (P < 0.01) .To add 0.05%, 0.10% and 0.15% mannatide can make the colibacillus and fusiform bacteria of colons go down 35.65% (P < 0.01 ), 86% (P < 0.01), 82.47% (P < 0.01) and 37.91%(P<0.05), 98.53% (P<0.01) and 94.66% (P< 0.01) separately/To add 0.05%, 0.10% and 0.15% mannatide to pig starter feed can have jejunum villus heights go up 17.70% (P < 0.05), 32.20% (P < 0.01) and 29.85% (P < 0.01) separately .10% and 0.15% mannatide can make the grand pit depth lower 16.82% (P < 0.05) and 19.20%(P < 0.05) . 0.15% mannatide can make the squuamous mucous crypt depth of colons far end upgrade 21.24% (P < 0.05) and the squuamous mucous cell desenity of coeca and colons upgrade 15.37% (P < 0.05) and 23.51% (P < 0.05) .To add 0.10% and 0.15% mannatide to pig starter daily gain can get the total proteolytic enzyme, trypsin and amylase activity of duodenum to increase 65.05% (P < 0.05) ,79.84% (P<0.01), 66.00% (P<0.01) ,52.24% (P<0.01), 33.06% (P < 0.05) and 2.34% (P < 0.01) .To add 0.10% and 0.15% mannatide to pig starter daily gain can make crude protein apperance digestion rate go up 6.29% (P < 0.05 ) and 5.41% (P < 0.05 ) .So mannatide has greatly improved the objects of the intestinal canal micro-biology envirnoment , stimulated the intestinal canal to grow up , strenghened the secernment of duodenum digesting enzyme and improved the digestion rate of small intestine.3. As to the immunityTo compare contrasted group, to add 0.05%, 0.10% and 0.15% mannatide to pig starter daily gain,serum IgG can upgrade 11.73% (P<0.05), 23.35% (P<0.01) and 25.18% (P < 0.01) separately , IgA go up 25.27% (P < 0.05), 52.75% (P < 0.01) and 71.43% (P < 0.01) as well as raise IgM tendency .But the difference is slight (P > 0.05) .To add 0.05%, 0.10% and 0.15% mannatide to pig starter daily gain can make T-lymph cell conversion rate raise 20.27% (P < 0.05), 55.41% (P < 0.01) and80.41% (P < 0.01) .So mannatide may strengthen the pig starters' functions of body liquil immunity and cell immunity.Finally, we have carried out an application test that"mannatide compoment pre-mixed feed "had been used to check piglet hog cholera anti-body level impact and feeding effect.As the results of the test have been shown,the pigs' hog cholera ahti-body levels,daily weights, survial rates ,feed conversions and economic profits in the tested group are better than those in the contrasted group in Jia-Hua Breeding Pig Co.,Ltd,Zhejing. The daily daily gain, 33lg, of testl,test2 and test3 are 400g> 424g and 429g.The feed conversion rates of testl,test2 and test3 are 2.10:K 2.01:land 2.01:1.The feed conversion rate , 2.27:l,in the contrasted group has been raised 7.49%^ 11.45% and 11.45% separately .The average death rate in the tested group is 1.67%, comparing with the death rate 15% in the contrasted group.The death rate is lower 13.33%.The costs of each kg gain testl,test2 and test3 are 4.32 yuan, 4.21 yuan and 4.26 yuan.Comparing with the costs, 4.61 yuan in the contrasted group,the percentages of the costs are lower 6.29%, 8.68% and 7.59%.The cost of gain of test2 are the lowest. |
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