| The growth of piglet was directly influenced by digestive tract development and immunity competence of body, then the raising rate of piglet involved. In suckling, because piglet's digestive tract was underdeveloped, its digestive function was unperfect, its passive immunity offered by the sow would weaken after three weeks' old, while its active immunity would bring into play low up to four_five weeks' old. Damaged intestinal configuration and function and lowered immunity of piglet were led by weaning , then more severe damages were resulted in early weaning. It is most essential that research and development of piglet feed which is cheap, can improve the digestive tract development and enchance immunity, can substitute milk or be used in early weaning . In vivo, the exeperiment was investigated to the effect of casein-, gluten, soybean protein hydrolysate on the digestive physiology and immunity of early weaning piglet in 21 days' old, and in vitro , on the gastrin secretion of gstric antrum and the transformation of lymphocyte in 10 days' piglet , in order to offer the accordings to develope the low cost and favorable effect of piglet feed substituting for milk or using in early weaning piglet. Part I Effect of casein, gluten and soybean protein hydrolysates on gastrin release of gastric antrumWith superfusion system, the research was investigated to the effect of protein hydrolysate on gastrin secretion of the gastric antrum of 10 days' piglets, at the same nitrogen quantity of the P-CM7 (10nmol/L). Results indicated that all the hydrolysates could promote gastrin release in the experimental group, and effect was distinct within the first 10-minute after stimulation began, then lowered gradually from the second 10-minute. The role of soybean hydrolysate in stimulating grastin release was stronger than that of the others, compared with the control, gastrin was respectively increased by 99.98%, 65.12%, 30. 45% and 9. 48% in four piglets fed by soybean hydrolysate, in feeding gluten hydrolysate group, it was respectively increased by 34. 66%,20.35%, 5.56% and 3.87% , in feeding casein hydrolysate group, which was increased by 107. 12%, 56.26%, 6.77% and 0. 72% respectively. Part II Effect of casein, gluten and soybean protein hydrolysates on the transformation of lymphocyte in 10 days' pigletThe effect of hydrolysate on the transformation of lymphocyte in 10 days' piglet was studied by 3H-TdR method. Results showed that each of the three hydrolysates could promote the transformation of lymphocyte induced by PHA in 10 days' piglet, though there existed obvious individual diversity among the four piglets. The soybean hydrolysate was of the highest action, then the gluten hydrolysate, finally the casein hydrolysate. Compared with each of the control groups, the stimulation indexes of the soybean hydrolysate group was increased 20.96 times , 11.65times, 0. 74 times and 0.39 times in four piglets respectively, of the gluten hydrolysate group, which was 10.96 times, 8.85 times, 2. 41 times and 0. 63 times respectively, and of the casein hydrolysate group, which was 1.82 times, 1.96 times, 1.54 times and 0.73 times respectively.PartHIEffect of feeding three protein hydrolysates on growth of early weaning pigletCompared with the control(weaning in 28 days' old), the slow appetite, diarrhea and growth delayed of early weaning piglet were conquered by feeding soybean hydrolysate or 3-casomorphin7, the growth of visceras be promoted in some degree , but the piglet weight change was not significant . Feeding gluten hydrolysate or casein hydrolysate can lead piglet diarrhea , and has no obvious effect on the weight changes of visceras . Compared with each other in the experimental groups, the effect of feeding soybean hydrolysate promoting visecras' growth is proir to 3 -casomorphin?, gluten hydrolysate and casein hydrolysate.PartlVEffect of feeding three protein hydrolysates on serum hormone level of early weaning pigletCompared with the control (weaning in 28 days' old), in 21 day's weaning piglet fe... |
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