Agrobacterium-mediated Npr1 Gene Conversion Anthurium Study

Anthuriun andreanum Lind., with attractive wattles, various leaves and colorfulblossom in all seasons, is a favorable flower in the world. Suffering from insects andvirus, it bears a great loss every year. NPR1, coloned from Arabidopsis thaliana, is akey regulatory gene of Systemic Acquired Resistance (SAR). Anthuriun andreanumLind. was transformed with Agrobacterium tumefaciens carring NPR1 to obtaintransgenic lines resistant to some insects and virus, which have made importantcontribution to zoology and economics.Peak buds was used to induce a lot of callus. Callus were cut in three different ways,and a number of planets with high quality were obtained. Thus, an efficientregeneration system of Anthuriun andreanum Lind. from callus was establishedduring a short time, which provided a good preparation for gene transformation.Callus were used as recipient of transformation. A series of transformation conditionswere optimized. After infected and co-cultured with Agrobacterium tumefacienscontaining transformation vector Pcambia1301 with NPR1 under 3 days pre-culturing,20℃,20min marination, 5 days co-cultured, callus produced 7 planets on selectionmedium supplemented with 400mg/L Cef and 50mg/L Hyg finally. Through PCRanalysis test, 7 planets were shown to contain NPR1 gene primarily.Results demonstrated that the broad-spectrum disease resistance could be achieved byAnthuriun andreanum Lind. mediated by Agrobacterium tumefaciens carring NPR1gene.