| The Arabidopsis NPR1 gene controls the onset of systemic acquired resistance (SAR), a plant immunity that is often activated after a local infection. Once established, SAR can provide the uninfected parts of plants a resistance to a broad spectrum of pathogens. To genetically engineer a broader spectrum of enhanced disease-resistance/tolerance in tomato plant, NPR1 was introduced into a tomato using the Agrobacterium tumefaciens mediator method. Twelve explants were selected by Km, PCR and Southern blotting results confirmed that three new plants were transformed. The transformed plants were assayed for resistance to CMV, confirmed that the resistance against pathogen infection in transformed plant was increased significantly, this work confirmed that the NPR1 could positively regulate the expression of tomato systemic acquired resistance and this gene can be used for engineering for resistance to disease. |
