Mouse Embryos Frozen

With the life science development ,as one of basis of life science,the laboratory animal has reached a great degree in our country as well. At present only the laboratory mouse and genectically engineered mouse are more than six thousand strains, and this number is increasing 600-800 per year. To be consistent with the development of the world life science, our country is constructing the "National Resource Library of Genectically Engineered Mice" to introduce and exploit these precious animals. It is very important to establish a rapid economic and reliable method for conservation of a valuble variety of strains in security.The technology of mice embryo cryopreservation is a reliable method on germplasm storage of the mice.We use ICR mouse as an example to systematically optimize the condition ofmice embryo cryopreservation with solution EG40 ES40 and EFS40 by rapideryopreservative and vitrified method. Other than straw, the cell freezing tubewas firstly adopted. Using EG40 and ES40,The development ratio reached66.4%x 66.7% and the implantation ratio reached 41.4% 50% respectively aftermaw EFS40 was used as solution to be vitrified .The development ratio andimplantation ratio reached 87.2%, 66.3% respectively. The difference is notemarkable compared to the control. We use EFS40 as vitrified solution and strawwas replaced by cell freezing tube, and the result is the same of the straw method.Nuthors compared one-step and two-step method, found the effect of EG40, ES40eryopreservative method is better than one-step method, and the difference isremarkable, while the different effect of EFS40 two-step vitrified method is notremarkable. Subsequently,with the straw and the cell freezing tube,we usedone-step and two-step method to vitrify the embryo of Lats gene knockout mice.The development ratio reached 90% 90.1 % 89.2%, 91.4% and the implantation rauo reached 50.0%, 47.1% 43.4%, 46.5% respectively after thaw. The difference is not remarkable compared to the control.With genetic assay,we can receive the result that the Lats gene of cryopreservative offspring did not lose.Taken together, the solution of ES40 and EFS40 are provided with more application than that of the solution of EG40.Especially, the vitrified method with cell freezing tube is even more simple and practical,but its result is outstanding...