Studies Of The Embryo Splitting Effection Before And After Frozen On The Mice

In this paper, the effect of different splitting-solutions, medium, splitting method, different refrigerating fluid and freezing method on the morulae and blastulae of mice were studied. The main results were as follows:1 First studied the effect of different splitting-solutions and different stage of embryo on the splitting. The success rate of morula splitted in 12.5% sucrose was higher than splitted in m-PBS(60.83% vs 51.67%,P<0.05), while there's no significant difference when the blastulae were splitted(P>0.05). Splitted the blastulae and morulae in m-PBS, the success rate showed significant difference (70.37%vs 51.67%,P<0.01), but there's no significant difference when they were splitted in 12.5% sucrose (P>0.05).2 No matter morulae or blastulae, the livabilily of half-embryos between the m-PBS and TCM-199-Hepes showed no significant difference (62.50% vs 63.64%;80.36% vs 82.76%,P>0.05); while cultured in m-PBS, there was significant difference between morulae and blastulae(62.50% vs 80.36%,P<0.05), and so as TCM-199-Hepes(63.64% vs 82.76%,P<0.05).3 The success rate of decompacted morulaes was higher than untackled(73.08% vs 51.92%,P<0.01), and there was significant difference in the development rate of demi-morulae (71.15% vs 46.15%,P<0.01)4 Demi-embryos were vitrified with VSâ… and VSâ…¡by the way of the OPS, after thawed, the livability of demi-embryos embed by agar was higher than untreated group in both two vitrified medium(90.16% vs48.28%;88.52% vs 44.83%, P<0.01), after cultured 48h, the developmental rate of demi-embryo about treated group was higher than that untreated group (61.82% vs 25.86%;61.11% vs 24.13%,P<0.01). In the group of agar , there was no significant difference between the VSâ… and VSâ…¡of livability and developmental rate(90.16% vs 88.52%;61.82% vs 61.11%,P>0.05), so as the group without tackled (48.28% vs 44.83%;25.86% vs 24.13%,P>0.05).5 No matter VSâ… or VSâ…¡, after vitrification, there was no significant difference between the rate of morphologically of morulae and blastulae(96.94% vs 95.96%;86.87% vs 84.38%,P>0.05).Vitrifide with VSâ… , there was significant difference between the morphologically rate of morulae and blastulae (96.94% vs 86.87%,P<0.01),and so as the VSâ…¡(95.96% vs 84.38%,P<0.01).6 Vitrified the splitted embryos of mice, the average livability was 40.48%, and the average livability of splitted embryo after vitrification was 81.63%, there showed significant difference between them (P<0.01).