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(60) Co ¦Ã-ray Irradiation Konjac Germination Bulbs Mutagenic Effect And Its Impact On Germplasm Improvement

Posted on:2003-10-09Degree:MasterType:Thesis
Country:ChinaCandidate:X D HuangFull Text:PDF
GTID:2193360065961949Subject:Crop Genetics and Breeding
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As a newly arisen industrial crop, AmorphophallusKonjaccontains vast economy development potential and better industrial advantages than others. Recently, Konjac industries is going to faster developing period, not only Konjac cultural area is constantly extending, but also Konjac industries is upgrading. So it requests that Konjac should be improved in its varietry germplasm and production qualities. The experiment was designed which Konjac germination hastening corm been induced to mutation by MCoY-ray. The evidence induced effects and other basic informations were obtained. These are importance to improve Konjac varietry germplasm in theory meaning and practice meaning. The study filled the blank of research field in konjac breeding.The conclusions were drawn as below.1.Germination hastening corm (contain water 81.25%) is optimal material when Konjac is induced to mutation by wCo Y -ray. The induced parameters were carried out: radiant energy range 0 ?0Gy; radiance IGy/min; middling optimum iradation dosage range 7?0 Gy; lethal dose 50Gy.2. The induced effects that could be measured were stimulation restraint and plant abnormality.The Konjac was stimulated in early characters which irradiated by Lower dose IGy 60Co Y -ray. The stimulation effects may be physiologic type irradiation damages. Restraint was dorminative induced effect. Lethal was extreme restraint effect. The irradiation could also caused Konjac plant lower or shorter andleaf abberation.3. The cytology effects were better evidence when the Konjac germination hastening corm irradated. Mitosis parameter minineucleus cell rate neucleus aberration cell rate, chromosome aberration cell rate were changed. There was conic between cytology effects and irradiation energy. It was linearity that minineucleus cell rate neucleus aberration cell rates chromosome aberration cell raterelatived one another. Cytology effects widly relatived to other characters induced effects, based on these, we could forecast characters induced effects laterly and choose fine characters.4. Iradiated germination hastening corm also caused biochemical effects .According the isoenzyme electrophoresis bands of esterase and polyhenol oxidase which evdently becomed smaller and weaker, the irradiation damages were concluded in structure and activity of enzyme.5. The ultilized mutations which plantleaf become bigger and plants could tolerent colder weather were found in the field. Ultilize bigger leaf plants to accumulate konjac corm nitrition , so the yield was improved. Ultilize anti-cold plants to postlong growth period , so the material was offered to enlarge planting.6.The change of konjac qualities were measured which caused by Y -ray. There were conies in parameters of dry matter cntain-glucomannan cntain and glucomannan viscosity changed with irradiation energe, linearity in parameters of alkaloid contain.7. By analyzing relationships that character parameters changed with irradiation energe, those were linearities in parameters of germinating rate, seeding rate. survival rate and alkaloid contain, cubic in leaf area parameter, conic in others character parameters. Based on main factor analyzing in 6 character parameters which contain anti-cold, yield and qualities, the model was estimated as below:y1=0. 776 q1+0. 978 q2+0. 914 q3+0. 990 q4+0. 937 q5+0. 514 q6 y2=-0.-612 q1-0.109 q2+0.383 q3+0. 115 q4-0. 337 q5+0. 84 0 q6yandicats the informations of yield and glucomannan qualities, y2 indicats the informations of alkaloid qualities. The model not only providing the basis to evalute induced effects,but also giving the reference to design breeding.
Keywords/Search Tags:60Co-ray, Amorphophallus Konjac Germination Hastening Corm, Induced Effects, Germplasm Improvement
PDF Full Text Request
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