Indirectly, The Dot-ppa-elisa Detection Of Ppv Antibody

Studies on the Detection of PPV Antibodies Using Indirect Dot桺PA桬LISA Fu Lizhi Wang Hongning et al College of Animal Science & Technology, Sichuan Agricultural University, Ya抋n China, 625014 Abstract This report describes Dot桽PA桯RP (PPA) enzyme-liked inimunosorbent assay was developed for detecting porcine parvovirus (PPV) antibodies. The optimum working concentration of PPA and PPV antigen was 1:160 and 1: 160 respectively. The optimum time of antigen sealing was 40 minutes at 10-25 Cent grand. The optimum working time of medium was 3桽minutes at 10-25 Cent grand. The detection limit of PPV antibodies by Dot桺PA? ELISA was 0.11 6ug per dot. The high specificity of Dot桺PA桬LISA was shown by the positive control test of porcine anti-PPV serum and the Cross-reaction tests with porcine anti-Pseudorabies serum, anti-Hog cholera serum, and anti-Japanese encephalitis serum. The positive serum from 10 experimental pigs inoculated vaccine two weeks after by Dot桺PA桬LISA and HI were positive, the highest titres of HI were 1024, the highest titres of Dot桺PA桬LISA was 4096. 110 33 serum field samples were detected PPV antibodies by Dot桺PA桬LISA and HI test. Among of 110 serum field samples, 81 were HI positive, while 29 were HI negative. Detecting the same serum samples by Dot桺PA桬LISA 93 was positive, while 17 were Dot桺PA桬LISA negative. The positive rate was 73.64% and 84.55% respectively., which shows that Dot桺PA桬LISA is more sensitive than HI (P<0.05). This study indicates indirect Dot桺PA桬LISA is a very important means for rapid diagnosis and epidemiological investigation of PPV infection. This study will provide a research basement for diagnosis products.