Aloe Vera Tissue Culture System

Study on technique system of tissue culture in Aloe Vera Du Wenping (College of Forestry and Horticulture, Sichuan Agricultural University, Ya抋n, Sichuan, China, 625014) Abstract The stem of Aloe Vera Linme was used for explant to establish the technique system of tissue culture. With Orthogonal design and analysis of variance, to find out the best medium and proceeding of culturing Aloe Vera. The results were as follows: ~Dstems ware sterilized in 75% alcohol and 0.1% mercuric chloride solution for 10 Seconds and 5 minutes well; ~ Iniation medium: H medium, exchanged well, with ZT2.Omg/L NAAO.O5mgIL and sugar 20g/L, is the best iniation medium; ?Subculture medium: The MS medium with the 2.Omg/L BA and 0.Olmg/L NAA was applied to the subculture medium, and the increasing coefficient in one month is 4.17, Using 35 days as subculture time, theoretically, the times of propagation is 1.59X 106 for one year; ~ Rooting culture: The rooting rate in the KC medium with 0.3mg/L IBM ACO.2% and sugar 2OgIL is 95.83%, the number of roots are 4.30, the length of root is 4.67cm; ~ Transplant: rooted Aloe or rooted Aloe in the rooting medium with AC or the transplant medium in perlite and vermiculite by 1 to 1, are all useful to root.