Vegetative Propagation And RAPD Analysis Of Aloe Vera L

Aloe vare L, growing mainly in the dry regions of Africa, Asia, Europe and America, is a member of the Lily family, it is very cactus-like in its characteristics. In the present study, vegetative propogation and analysis of RAPD of Aloe vare L were carried out. The primary results could be summarized as follows:1. The genome DNA from 13 families of Aloe were succesfuly extracted by using the improved CTAB method. And the analysis of genetic diversity of these families in Aloe vera L. were carried out by RAPD molecular markers, which may help to provide the theoretical base for breeding. 16 primers were screened from 206 random oligonucleotide primers and 39 polymorphic loci were obtained from PCR of 17 primers. 35 of the primers could amplify polymorphic bands. Out of 123 amplified bands, 78 bands are polymorphic. Every primer could amplify 1 to 9 polymorphic bands, with an average of 4.94 bands.2. Significant differences were observed among the different media, auxins, culture temperature, culture light. The regenerated plantlet percentage was significantly higher under medium MS, under 25 ℃ and 12h light/12h dark than that under medium N6, 20℃ and 30℃and 24h dark. No difference was observed between under 20 ℃ and 30℃, as well as 12h light/12h dark and 24h light. On the basis of the results, a system for vegetative propogation of Aloe vera L was established and applicated in the farm practice.