Research On The Relationship Of Tlr4 Genotypes With Expression Level Of Nf-κb And Associated Genes In Xinjiang Brown And Holstein Cattle

Nuclear factor-κB (NF-κB) has intimate association with inflammation, immune response, differentiation and apoptosis. In NF-κB passway, TLRs play an important role in the events of inflammation and immune response. Based on our previous investigations on the subclinical cow mastitis and correlation of TLR4 E3+2021 genotype in Xinjiang brown cattle and Chinese Holstein, we measured the transcriptional level of nine major genes (NF-κB1, NF-κB2, RelA, c-Rel, RelB, IKKα, IKKβ, IKKγ, IκBα) involving the NF-κB passway among AB and BB genotypes. The results revealed that the transcriptional levels of NF-κB1 and IKKαin Chinese Holstein were significantly higher than those in the BB genotype in Xinjiang brown cattle (P<0.05). The transcriptional levels of RelA, RelB, IKKβ, IKKγand IκBαin Chinese Holstein were extremely significantly higher than those in the BB genotype in Xinjiang brown cattle(P<0.01). The transcriptional levels of IκBαin Chinese Holstein were significantly higher than in Xinjiang brown cattle in the AB genotype(P<0.05). In the studies on Xinjiang brown cattle, we observed that the transcripts of AB genotype of RelB, IKKγand IκBαwere significantly higher than those of BB genotype(P<0.05). While the transcriptional levels of the nine genes between AB and BB genotype were no significantly difference in Chinese Holstein.Taken together, the variation of the mRNA transcriptional levels of NF-κB1, RelA, IKKβ, IKKγand IκBαwithin three different genotypes between Xinjiang brown cattle and Chinese Holstein was probably associated with the different mastitis incidence in two breeds. Moreover the high transcriptional levels of RelB, IKKγ, IκBαmay be one of the causal to the low SCS in AA genotype in Xinjiang brown battle.