Study On Tissue Culture Of Populus Deltoides Cl. 'danhong' And Eucalyptus Grandis × E.urophylla

Populus deltoides CL.'Danhong'was selected by the Chinese Academy of Forestry and Eucalyptus grandis×E.urophylla was selected by the Forestry Research Institute of Guangxi. The two superior clones were fast growth and high economic value.They had been widely cultivated and were main clones of construction of commercial forests in south of China.The major objective of this paper was to establish the optimal regeneration systems for Populus deltoides CL.'Danhong'and Eucalyptus grandis×E.urophylla in vitro. Establishment of high-efficient tissue culture reproduction systems of Populus deltoides CL.'Danhong'and Eucalyptus grandis×E.urophylla provided materials and basic means for multipurpose studies, such as selection and cultivating of insect-resistant and cold-resistant of genetic transformation, to expand its cultivated area. The results are as following:1. The significant factors of germination axilary buds is 6-BA, NAA and medium. The optimal induction medium are MS+6-BA0.3-0.5mg/L+NAA0.1mg/L and WPM+ 6-BA0.3-0.5mg/L+NAA0.1mg/L.WPM+6-BA0.5mg/L+KT0.3-0.5mg/L +NAA0.5mg/L is available to stem elongation inducement. NAA is considered the significant factor, KT is regarded the small factor.1/2MS+IBA0.2mg/L+ NAA0.3mg/L and B5+IBA0.2-0.3mg/L+NAA0.3mg/L are available to roots culture inducement,rooting rate being up to 100%.The sterile young plants have been transplanted successfully,the survival rate was up to 80.0%.2. The optimum medium for inducing callus from leaves was MS+2,4-D0.2mg/L+ 6-BA1.0mg/L,but callus rate is only 57.5%.1/2MS+IBA0.2mg/L+NAA0.3mg/L is available to induce callus from stem without axial bub, callus rate is being up to 82.26%. MS+6-BA1.0mg/L+KT0.5-1.0mg/L+NAA1.0mg/L is available to induce callus from roots. The most optimal callus multiplication medium was MS+BR 0.1 mg/L +NAA0.1mg/L.When the cytokinin concentration higher than the auxin concentration is conducive to callus differentiation of adventitious buds.WPM+6-BA0.2mg/L+ TDZ0.001-0.003mg/L+KT0.5mg/L+NAA0.05-0.10mg/L is available to induce adventitions buds from callus. 3. The concentration of the NAA:ZT equal to 2:5 can significantly improve the multiplication of buds.Thus, improved MS+ZT0.3mg/L+NAA 0.5mg/L is available to enhance the multiplication quotiety of adventitious shoots.The mineral is adjusted the concentration of Ca2+.Mg2+Fe2+and Mn2+on which the basis of the MS salt in order to reduce the proliferation of yellow buds.Improved 1/2MS+NAA1.5mg/L+IBA 1.Omg/L is available to roots culture inducement, rooting rate being up to 100%. The sterile young plants have been transplanted successfully, the survival rate was up to 85.0%.