Digital Cell Micro-injection Analyzer Developed And Pull The Needle Instrument Design

Cell Microinjection is one of the technologies widely used in the domain of Cell Engineering. However, the domestic equipment of cell microinjection now is totally depended on the foreign companies whose products are very expensive and inferior in cell distortion, flow stability and productivity. Furthermore, their products haven't realized the automation of cell microinjection.Based on the Digital Microfluidic Technology, a cell microinjection device is designed, drived by piezoelectric actuation. Digital microinjection displacement testing experiment system is builded to research the influence of drive wave, voltage, frequency, gradient degree and impacted force. Furthermore, the cell microinjection experiment system is builded to research the effect of the microinjection device. In addition, a design scheme is presented to develop a micropipette puller, in order to fabricate good cell injection pipette.By controlling the change of drive wave, voltage and frequency with soft, the cell microinjection device can well realize not only the rapid movement of pipette's going ahead and back, but also the microinjection manipulation. The device avoids direct adjustment to the structure, which makes it easy to realize the automation of cell microinjection. The results indicate that the device makes less distortion to the cell when penetrating into it. Also the device has a better flow stability and a higher resolving power of injection. The micropipette puller system designed in the paper can fabricate good cell injection pipette with high stability. The results indicate that it's better to increase the heat voltage and reduce the heat time and length in a proper range.