Immobilized Lipase Catalytic Swida Grease The Synthesis Of Biodiesel

Energy resources increasingly straining causes for people's concern. Therefore, as the renewable substitute energy, biodiesel was taken into consideration by more and more country. At present, compared with developed countries, the development and utilization of biodiesel is relatively backward in China. It is a pressing problem to be solved of researching Biodiesel. Swida wilsonian is a kind of valuable oil tree, as an ideal biodiesel feedstock, it has good economic and social benefits.In the paper, in order to find a low-cost,easy to operating and environment-friendly route of synthesizing biodiesel,and make biodiesel from Swida wilsonian oil into industrialization. The conclusions were showed as follows:The extraction methods of Swida wilsonian oil were researched. First using Complex Enzyme to digest pulverizing Swida wilsonian seeds, second using Soxhlet continuous reflux to extrat Swida wilsonian oil. The extraction rate of five different solvents was that petroleum ether> n-hexane>ether>ethyl acetate>ethanol.Economically, petroleum ether was the suitable solvent. The optimum extraction process was achieved: pectinase, a-amylase and composite cellulose enzyme complex used to digest, Swida wilsonian seeds to water ratio of 1:0.67,pH=7.5, petroleum ether as solvent, temperature 50℃, extraction time 3 h, Swida wilsonian seeds to petroleum ether ratio of 1:15. Under this condition, the extraction rate was 27.8%, compared with no treatment of enzymethe, the extraction rate was more efficient.Acid degumming method off the phospholipid in Swida wilsonian oil, reduce acidic value with solvent extraction method. This article examined the single factor of reaction temperature, water content, hydration time effect on phospholipid content. Investigated the amount of anhydrous ethanol, the extraction times and operating time work upon acidic value. Get the best optimal conditions:reaction temperature 70℃, the amount of water was 4% of Swida wilsonian oil, hydration time 40 min. Under this condition, Swida wilsonian oil phospholipid content reduced to 12.98 mg/kg. The ratio of ethanol and oil (V/W) was 2:1, extraction four times, operating 15min, Swida wilsonian oil acid value dropped to 0.97 (KOH)/(mg/g).The project firstly studied the transesterification of Swida wilsoniana oil to biodiesel with the catalyst of immobilized lipase Lipozyme TL IM in the saturated solution of MgCl2. The results indicated that in the MgCl2-saturated solution system (1) the maximum yield of biodisel reached 86.5% after 8 hours and (2) the optimal conditions were:3:1 methanol/oil molar ratio,20% of Lipozyme TL IM based on Swida wilsoniana oil weight and agitation speed with 150 r/min. As is known, the traditional method of transesterification works by three successive additions of methanol and tertiary butanol solution-containing system. We also compared the proposed method to the traditional method. It was found that the MgCl2-saturated solution system could effectively solve the problem of immobilized lipase deactivation. Moreover, our method possesses the advantage that it was of better lipase stability, higher reaction efficiency and Low production costs. Therefore, it's expected to be a new process for biodiesel production.Chemical constituents of biodiesel from Swida wilsoniana oil were researched. Analyzed by GC-MS and the conclusions were that the content of methyl oleate is 53.67%, methyl stearate is 17.36%, methyl linoleate is 12.35%, methyl palmitate is 14.35%.