New Method Of Nano-tio <sub> 2 </ Sub> For The Photocatalytic Oxidant Light, Chemical Luminescence

Two Parts are included in this thesis.In the first part of this thesis,the main principles and the developments of chemiluminescence(CL) and some coupling techniques with CL are reviewed.In the second part,we mainly focused on the study of principles and analytical applications of Co²⁺-doped TiO₂ nanoparticles in chemiluminescence system.Chemiluminescence analysis,based on the emission during some chemical reactions,is a technique for the determination of trace species.Due to its rapid analysis,high sensitivity,wide linear range,simplicity and relatively low cost,chemiluminescence analysis has received much attention over analytical chemistry.It has been widely used in chemistry,biological engineering, food,medicine,environmental chemistry and so on.With the development of chemiluminescence analysis in various fields,some new luminescence analysis methods appearance such as luminescence imaging promote luminescence analysis' development and application greatly except the traditional analysis method and technology development rapidly.In recent years,due to various advantageous properties of nanoparticle,which lave been found to offer new opportunity for rapid and sensitive CL analysis method.The purpose of this thesis was to study the conditions,characteristics and analytical applications of the photo-inducing chemiluminescence by the combination of nanotechnology and chemiluminescence system of luminol.In this thesis,we not only developed a novel photo-induced chemiluminescence(PCL) method in situ,but also proposed a new idea of regulating CL based on nanotechnology by the combination of Co²⁺-doped TiO₂ nanoparticles and chemiluminescence system of luminol.At the same time,we study the conditions,characteristics and analytical applications of the photo-inducing chemiluminescence with Co²⁺-doped TiO₂-luminol system as representative.The works are included in the followings:1.Study on Photo-induced Chemiluminescence In-situ with Co²⁺-doped TiO₂ Nanoparticle as Photo-catalytic Oxidant and Its ApplicationSuperoxide anion was produced on the surface of Co²⁺-doped TiO₂ nanoparticles by the photo-inducing effect and the characteristics of adsorption and desorption of superoxide anion on the surface of Co²⁺-doped TiO₂ nanoparticles were studied with Co²⁺-doped TiO₂ nanoparticle as photo-catalytic oxidant.At the same,the effects of the electrical double layer formed on the surface Co²⁺-doped TiO₂ on the CL signal were studied.This photo-inducing chemiluminescence(PCL) signal on the surface of Co²⁺-doped TiO₂ nanoparticles had the time and space resolved performances due to the characteristics of adsorption and desorption of superoxide anion and electrical double layer effect on the surface of Co²⁺-doped TiO₂ nanoparticles.Based on these findings,we not only developed a novel PCL method in situ,but also proposed a new idea of regulating CL based on nanotechnology.Under the optimum experimental conditions,the enhancing PCL signal was linear with glibenclamide concentration in the range of 2.0×10^-8～1.0×10^-6g/mL and this new PCL method offered a 6×10^-9 g/mL detection limit for glibenclamide.2.A Highly Sensitive Photo-induced Chemiluminescence Method for Procaterol Hydrochloride with Co²⁺-doped TiO₂ Nanoparticle as the Photo-catalytic OxidantIt was found that procaterol hydrochloride could be effectively pre-concentrated on the surface of Co²⁺-doped TiO₂ nanoparticles and procaterol hydrochloride on the surface of Co²⁺-doped TiO₂ nanoparticles could form the active oxygen-based species in alkaline by the photo-inducing effect. Then,based on the sensitive CL response performances of active oxygen-based species to luminol CL reaction system,a new and high sensitive PCL method for the determination of procaterol hydrochloride was developed.Under the optimum experimental conditions,this new PCL method offered a 4×10^-11g/mL detection limit for procaterol hydrochloride and the enhancing PCL signal was linear with procaterol hydrochloride concentration in the range of 8.0×10^-10～1.0×10^-8g/mL.The presented method was successfully used to detect procaterol hydrochloride in urine samples.