Synthesis And Application Of Multiple Response Fluorescent Probe For Detection Of Superoxide Anion And Hydrogen Polysulfides

Recently,some evidence have shown that H2 S is the third endogenous gaseous signaling molecule after CO and NO. H2 S isplaying the important physiological and pathological roles inthe cardiovascular system and the nervous system. But more and more evidence have shown that dihydropolysulfides(H2Sn) is the actually signaling molecules in physiological activity, and H2 S is the emissions of H2 Sn in physical activity.Superoxide anion radical(O2?-) is one of the most important reactive oxygen species(ROS) in cells, whose formation with diverse physiological and pathological processes.At the same time, ROSstimulate the cell to produce H2 Sn in mitochondria. We report two near-infrared fluorescent probesto detection of reactive oxygen species(ROS)and dihydropolysulfides(H2Sn)in cancer cells and mitochondria.3-nitrophenol was selected as dihydropolysulfides(H2Sn) reaction site, benzyl chloride for mitochondrial fixation and the biotin was selected as the cancer cell targeting group. The probe applied to detect exogenous and endogenous dihydropolysulfides(H2Sn)in living cells, and realized the cancer tissue fluorescence imaging in vivo.This dissertation consisted of six chapters summarized as follows.1. We introduced the design of the mechanism of fluorescence imaging, cyanine fluorophores and fluorescent probes. Reviewed the publishedfluorescent probes to detecting superoxide anion and hydrogen sulfide, more and more development of near-infrared fluorescent probe to detect of superoxide anion and hydrogen sulfide have far-reaching significance inthe biological development study.2.We described the specific steps of design and synthesis of new multi-response near-infrared fluorescent probe, and introduced the detection mechanism of fluorescent probe to detect superoxide anion and hydrogen sulfide.3.We have tested the novel fluorescent probes to detected superoxide anion and dihydropolysulfidesin vitro, using an ultraviolet-visible spectrophotometer and fluorescence analyzer to evaluate under different pH conditions the stability, selectivity and chemical kinetics of fluorescent probe.4.We have detectedthe superoxide anion and dihydropolysulfides with the fluorescent probes in cells and in vivo by the laser scanning confocal microscope and vivo imaging system.And we detected the superoxide anion and dihydropolysulfides with the fluorescent probe in the mitochondria. Then the fluorescent probe was successfully applied for visualizing superoxide anion and dihydropolysulfidesin tumor mice models.5.Summarized the main content of the paper.