Semiconductor Nanoparticles Modified Electrode Electrochemical Luminescence Characteristics Of Study

Electrogenerated chemiluminescence (ECL) analysis has received considerable attentions sinceit not only owns the advantages of the conventional chemiluminescence analysis, such as excellentsensitivity, wide linear range and simple optical requirement for instruments, but also offers someadditional advantages. For example, the ECL reaction can be easily regulated and manipulated bythe applied potential. In specially, ECL has great development after combination of the chemicallymodified electrode and ECL. Chemically modified electrode technology was brought into ECL,which aims to improve the properties of the electrogenerated chemiluminescence. Chemicalmodified electrode breakthrough ranges that traditional electrochemical only limited to study on bareelectrode or interface of electrolyte. Opening a new field of artificial contronning electrode surfacestructure from chemical condition. By tailoring the molecular on the surface of electrode, theanticipant reactions will occurre on the surface of modified electrode. In recent years, thepreparations and synthesis of the nano-structured materials have attracted tremendous interest and anumber of approaches for preparing the functionalized nanoparticles have been applied in thedifferent fields of analytical chemistry. The purpose of this thesis was to regulate the reaction ofluminol ECL system and improved their analytical performance by nano-technique.Two parts are included in the thesis: review and the research paper.In the first part of this thesis, the definiens, property, future prospect of ECL analysis arereviewed. Then, the common system of electrogenereted chemiluminescence and the principle ofECL are mentioned. In addition to these, the combination with the nano-technique and the futureapplication of the nanoparticles modified electrode in the ECL analysis are also given in this part.The second part of this thesis is experiment part, including:1. Electrogenerated Chemiluminescence (ECL) Detemination for Gentamicin Sulfate withNano-ZnO Modified Electrode.ZnO nanoparticles were synthesized by two steps and modified the surface of graphite electrodeby drop-coating method. It was found that, Compared with bare electrode, gentamicin sulfateshowed stronger ECL enhancing effect for the weak ECL signal of electro-oxidation of luminol,while nano-ZnO modified electrode was used as work electrode. The reason that caused strongerenhancing ECL was ascribe to good micro-environment that ZnO nanoparticle provided on thesurface of modified electrode. The enhancing effect of luminol-based by gentamicin sulfate was improved in the presence of the micro-environment. And based on this, we proposed a highlysensitive ECL analytical method detecting gentamicin sulfate. Under the optimum experimentalconditions, the gentamicin sulfate concentration in the range of 4.0×10～(-10) to 7.0×10～(-8) g mL～(-1) wasproportional to the enhancing ECL signal and offered a 3×1O～(-11) g mL～(-1) detection limit for gentamicinsulfate.2. Electrogenerated Chemiluminescence (ECL) Detemination for Isoniazid with Self-assemblyMultilayer Nano-TiO2 Modified Electrode.The TiO₂ nanoparticles were immobilized on the surface of chitosan film coating graphiteelectrode by the self-assembled technique. It was found that, Compared with bare electrode,isoniazid showed stronger ECL enhancing effect for the weak ECL signal of electro-oxidation ofluminol based on effect of catalytic activity and preconcentration of nano-TiO₂ on the surface ofmodified electrode, while nano-TiO₂ modified electrode was used as work electrode. Under theoptimum experimental conditions, the isoniazid concentration in the range of 8.0x10～(-10) to 9.0×10～(-7)gmL～(-1) was proportional to the enhancing ECL signal and offered a 4.0×10～(-11)g mL～(-1) detection limit forisoniazid, relative standard deviation of 2.8%(n=11).