The Investigation And Analytical Application Of Luminol Electrogenerated Chemiluminescence System With Chemically Modified Electrode

Abstract: Two parts are included in this thesis. In the first part of this thesis, the progress in Electrogenerated chemiluminescent (ECL) and the analytical application of the chemically modified electrode in ECL are reviewed; In the second part of this thesis, we mainly focused on the study of the analytical application of the chemically modified electrode in luminol ECL system in order to improve the analytical performances of luminol ECL system and extend the range of analytes detectable with luminol based ECL system.Electrogenerated chemiluminescence is the luminescence generated by relaxation of exited state molecules that are produced during an electrochemically initiated reaction in the near surface of the working electrode. ECL is an important and powerful detection method in analytical chemistry in recent years. This method not only retains the advantages of the conventional chemiluminescence (CL) analysis, but also offers some advantages over more conventional CL. ECL analysis, compared with CL analysis, offered the more alternative potentials to regulate the ECL reaction for analytical purpose.Since the luminol-based ECL phenomenon was firstly observed by Harvey in 1929, the ECL was paid much attention by the analysts, and a lot of analytes had been detected with luminol ECL system. ECL proved to be useful for analytical application and increasing investigations resulted in highly sensitive and selective detection methods. It has been applied successfully in many fields, such as life science, clinical medicine, environment, immunoassays and DNA probe analysis etc.The chemically modified electrode represents a modem approach to electrode. It relays on the placement of a reagent onto the surface of it, to impart the behavior of that regent to the modified surface. Such deliberate alternation of electrode surfaces can thus meet the needs of many electroanalytical problems, and may form the basis for new analytical applications.Our research works are divided into two parts. Firstly, we focused our interesting on the combination of the chemically modified electrode technique with luminol ECL...