Ultrasonic Preparation Of Paclitaxel Solid Lipid Nanoparticles

Solid lipid nanoparticles (SLN) is a promising drug delivery system with solid natural or composite biocompatible lipid as drug carriers, which has the advantages of high physical Stability, slow speed of drug leakage and low toxicity etc. The SLNs was successful prepared by an ultrasonic-solvent for oral administration, which reduces administration costs and facilitate the use of more chronic treatment regimens.An HPLC method was the assay of formulation (methanol:methanol:water=2:9:10).The sephadex column chromatography were developed for entrapment efficiency. The RP-HPLC-sephadex separation method was simple, fast, accurate and the results of separating by sephadex column was sTab.le, good reproducibility.The paclitaxel loaded solid lipid nanoparticles were prepared by an ultrasonic-solvent emulsification technique and solvent emulsification, which has the advantages of high entrapment efficiency. The particle size distributions of the paclitaxel-SLN were examined by photon correlation spectroscopy (PCS), the mean particle size was about 300nm. The zeta potential was -24~30mV. The dynamic dialysis was performed to investigate the in vitro release. The results of the release experiments accorded with correlate equation.An HPLC method was developed for the determination of paclitaxel in the plasma. Oral and intravenous pharmacokinetic behaviors of paclitaxel suspension and two paclitaxel-SLN were investigated. The pharmacokinetic parameters showed that the relative bioavailability of paclitaxel in SLNs was significantly increased compared with that of a paclitaxel emulsion.