Banlangen Extraction Process And Quality Standards

Isatidis is the xero- root of Isatis tinctoria,having been widely cultivated in china.It's one kind of orthodox heat-clearing and detoxicating medicinal materials in our country,having many effectiveness of Anti-virus,Antiendotoxin,Antiinflammatory,Anticarcinogenic and so on.It's reported that these effectiveness come from water-solubility amino acids,polysaccharides and liposolubility Indirubin,Indigotin- the effective component in Isaris.But now the extraction technology is the main technology of extraction artwork,which obviously makes tremendous expenses of the liposolubility effective component.The experiment investigated the extraction technology systematically,also used HPLC to establishe its quality standard.With the content of Indirubin -the effective component in Isatis as index,the influencing factors(The density of alcohol solvent,the ratio of material and solvent and the extract time and so on)were studied by the orthogonal design.The best extract technology was accepted,it was as follows:70%alcohol as solvent;the ratio of material and solvent 1:12;extracting three times with 1h for each time.A proper quality standard was established by HPLC to determine the content of Radix Isatidis in Indirubin and Arginine.the content of Arginine -the effective component in Isatis had been determined on the mobile phases consisted of methanol-water(80:20 0.005mol/L C₈H₁₇NaO₃S and 0.02mol/L potassium dihydrogen phosphate)with the wavelength of detector 206nm.The content of Indirubin -the effective component in Isatis had also been examined on the mobile phases consisted of methanol-water(80:20 0.0%diethylamine)with the wavelength of detector 289nm.The results showed that the content of Arginine was 9.79mg/g and Indirubin was 4.12μg/g in Isatidis preparation.And the TLC method had also been used to discriminate Arginine and Indirubin with the developing solvent consisted of n-butanol- glacial acetic acid-water(19:5:5)and N-hexane- methylene chloride- acetone- acetone(5:4:1).In the field of pharmacy tests.Formulation process of granule was also investigated to provide a basis for the large scale production.