Bu Yang Huan Wu Tang Polysaccharides, Flavonoids, Volatile Oil Extraction And Analysis

Buyanghuanwu Decoction (BHD) was a classical prescription with the fuction of filling vigor and activating blood. It had prominence curative effect on apoplexy and it's sequela with weak vigor or blood blocked. In this article, the extraction technology of three effective chemical parts in BHD was studied by MAE technology, the concentration of polysaccharide and flavone in BHD were determinated by spectrophotometry, the constituents of volatile oil in BHD was analysised by GC-MS. The dynamic change of chemical ingredients in BHD was discussed primarily before and after drug pairing.The optimal extraction technology of polysaccharide in BHD was: the ratio of drug to water l:7,microwave power 390w, extraction time 20min, the yield of polysaccharide was 7.75% in this condition. The optimal extraction technology of water-soluble flavone was: the ratio of drug to water1:6, microwave power 390w, extraction time 15min, the concentration of water-soluble flavone was 1.29mg/mL and total flavone was 6.96mg/mL in BHD. The volatile oil in BHD was extracted by microwave radiation and SDE method with yield of 1.40%. The volatile oil in every single drug, drug pair and BHD was analysised by GC-MS, there were fifty-nine compounds in volatile oil of BHD, the main composition were alkyls, aromatichydrocarbons,hydroxybenzenes and carboxylic acids. Especially, benzaldehyde had the highest concentration of 22.35%. Compared with single drug, the concentration of terpene compounds in volatile oil were changed greatly. It showed that MAE had advantages of less time consuming, high effect, less solvent and no pollution compared to general extraction methods.