Application Of High Performance Liquid Chromatography - Mass Spectrometry In The Analysis Of Transgenic And Non - GM Food Components

High performance liquid chromatography(HPLC) has become one of the fastest growing, the most affected and the most widely used modern analytical methods. In terms of relative to other chromatography, HPLC can achieve fast separation test. High performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) has the characteristics of high selectivity, sensitivity, and throughput. It has been applied to a wide variety of studies in pharmaceutical analysis and life sciences. However, in high performance liquid chromatography-fluorescence or ultraviolet detection(HPLC-FLD/UV) analysis works, some of the target analytes have no chromophores or weak fluorescence intensity, which could not be using the high sensitive fluorescence or ultraviolet detection mehod. By being reacted with the fluorescent derivatization reagent and getting derivative products with the high activity of fluorescent. After separation highly effective HPLC-FLD/UV, the quantitative and qualitative determination of target analytes in the sample could be realized.In recent years, the use and release of genetically modified foods(GMOs) has been an issue of intense public concern and, in the case of foods, products containing GMOs or products thereof carry the risk of consumer rejection. In this article, we have providing a promising approach to establish an objective evaluation for transgenic foods. Microwave assisted extraction(MAE), microwave-assisted extraction-derivatization(MAED) and ultrasonic-assisted extraction-derivatization(UAED) are novel and efficient sample pretreatment technology. In this paper, we have been used the three effective sample pretreatment technology successfully extract plant growth regulator, sugars and amino acids in transgenic modified and nontransgenic modified foods, providing an important basis for objective evaluation of transgenic modified food by the comparison of composition and content. MAE, MAED and UAED have many advantages such as simple, low consumption, high recovery and high enrichment factor(EFs) and the extraction procedure is very fas which are greatly shorten the pretreatment time before and obtained the good experimental results. In this article, novel hyphenated method based on MAE, MAED and UAED have been established for the efficiently determination of transgenic and nontransgenic foods by HPLC-MS/MS detection. The hyphenated methods are verified for the feasibility of detection. The specific work contents are as follows:Chapter one: Introduction The progress of the application of sample pretreatment technique, HPLC, HPLC-MS/MS analysis and the the main research content.Chapter two: The introduction of a novel sample pretreatment technique, microwave assisted extraction and derivatization prior to HPLC-FLD-MS/MS applied for sensitive, accurate and rapid determination of the trace levels of 6 kinds of acidic plant growth regulators in transgenic and nontransgenic edible oils.Chapter three: The introduction of a novel sample pretreatment technique, microwave assisted extraction prior to HPLC-UV-MS/MS applied for sensitive, accurate and rapid determination of the trace levels of 5 kinds of carbohydrates in transgenic and nontransgenic edible oils.Chapter four: The introduction of a novel sample pretreatment technique, ultrasonic assisted extraction and derivatization prior to HPLC-FLD applied for sensitive, accurate and rapid determination of the trace levels of 20 kinds of amino acids in transgenic and nontransgenic edible oils.Chapter five: Conclusion and future prospect.