The Development Of Flow Injection Analysis System And Amino Acids For Rapid Detection Research

Virtual Instrument is one kind of new instrument that has developed step by step along with the progress of computer technology since 1990, which is one kind of instrument that collects data and analyzes data by computer, it has visual panel. This thesis discussed how to design one set of chemical Virtual Instrument to fulfill the need of Flow injection Analyze.The Project was designed by comparing the theory and application area of all kind of instruments based on researching the progress of develop, the theory of design and the method of execution of visual instrument in and out of our country.This visual instrument works on common circuit of Peripheral Component Interconnect, it is made up of data acquisition card, peristalsis pump, injecting sample valve and photometer. The data acquisition card has the function of AD, DI and DO. The peristalsis pump and injecting sample valve were used to deal with liquid. The photometer was used as detector. The software was programmed with Visual Basic 6.0, it function was designed according to the need of FIA.The interface of the system is friendly. It is simple and convenient to operate the system. The function of the system is perfect .The system can work stably and sensibly. Because it is made up of integrated component, it is convenient to maintain it. Other apparatus also can use the hardware and software of this system.At last, this thesis discussed the research of method of determine the total content of amino acid quickly.Employed with ninhydrin and FIA, the acidity, the velocity of carry liquid, the volume of tube of gathering sample, the time of heat the liquid and other parameter that affect the result of determining of amino acid were discussed and the best condition was gained: pH=5.4; =570nm; the velocity of carry liquid and reagent is 2.0ml/min; the content of ninhydrin is 0.2%;the time to heat the liquid is 6min.It has a good linearity of the content of amino acid and its absorbency when the content of amino acid is between 0~2.0 g/ml.The precision of this determine method is good, and the wastage of reagent is a little. It is easy to apply the method and it can shorten the time of determining by a long way. According this method, we can design the special instrument to determine the total content of amino acid and apply it to quality control in factory.