Polyphenols Of Qualitative And Quantitative Analysis Methods And Macroporous Resin Purification Process Conditions Are Optimized

Tea polyphenols (TP) are one kind of materials with great physiological activities , such as anticancer or antitumor , antihyperlipidemia and antibacterial or antivirus , which decides their great value in medical research . For the moment, the products of tea polyphenols are largely crude , which must be separated and purified to meet the demand of food or medical industry . Some research was carried for the purposes of getting tea polyphenols with medical injection standard purity . The purpose also involves valuing the quality of tea polyphenols and finding optimal process to separate and purify tea polyphenols . Through comparing the chromatograms of the samples with the chromatograms of references , tea polyphenols is qualitatively analyzed by high-performance liquid chromatography fingerprints . Tea polyphenols are also quantitatively analyzed by tritrametry with KMnO4 , spectrophotometry and high-performance liquid chromatography , which is the basic research of building two kind of new methods to analyze tea polyphenols quantitively . The parallelism and consistency of the analysis data shows the feasibility of the methods . In this article , basic research of separate and purify tea polyphenols by macropore adsorption resin is carried , too . Orthogonal experimental design is also applied to determinate the optimal separation/purification process conditions, with HPD-600 macropore adsorption resin as carrier, H^O/ethanol as solvents . And the optimal process is as follows . The solvent to dissolve samples is HaO/ethanol, and the ratio is 1:3 ; the concentration of solvent used for elution is 70% ; the velocity of elution is 5 ml/min .