| The work used modern analysis technology(Supercritical CO2 extraction, macroreticular resin) on extraction antitumor activity compound CPT.At first, development of CPT in country and oversea was introduced, including conformation and resource distributing,main chemical ingredient,structure and physical chemistry property, relationship between structure and anti-tumor activity mechanism, CPT of modification, CPT of extraction etc. Particnlarly modern extraction and seperation technology of Chinese traditional madicine,such as SCF and macroreticular resin were introduced.Condition of Determination CPT content by HPLC was emphasized. The results rum out to be an regressive equation. CPT content in camptotheca acuminata fruit extracted by SCF was determined and gained good recurrently.The results showed close loop form of CPT was stable to light but instable to heat. The experiment condition of extraction of CPT by SCF and separation by macroporous resin were optimized.Optimization experiment condition by SCF find out to be:pressure 35Mpa,Tempreture 55 癈,dosage CC>2 80kg(co2/kg(materiai)-The optimization condition by macvoporous resin is:chloroform/alcohol(l:l), PH=3, velocity l.Oml/min.Extration of volatile oil from camptotheca acuminata fruit by SCF was introduced for the first time.Colourless clarity oil which scent identical to camptotheca acuminata fruit was gained.Ratio was 1.60%, 4.6 time as that by vapor distillation.Rapid cheapness CPT'S industrialization produce was also low poison, little pollution and effective method for explored in this paper. |
