Study On Extraction Of Collagen From Gi Er Li Go Mongolia Bovine Bone And Its Physical And Chemical Properties

Taking the Jierlige bovine bone in Erdos of Inner Mongolia as raw material, acetic acid and pepsase to extract I-type collagen in bovine bone was used in this paper,meanwhile physicochemical property was researched. Based on single factor experiment, orthogonal test was adopted in this paper to screen the optimum extraction parameter for bovine bone collagen, the enzymatic additive amount is 4%, the extraction period is 48h, and the extraction ratio of feed liquid is 1:10, by this time the extraction ratio reaches to highest, which is 16.92%.The collagen extracted from SDS-PAGE gel electrophoresis represented bovine bone is I-type collagen. The USA (Ultraviolet Spectrum Analysis) result showed that the maximum absorption peak for bovine bone collagen is 230mm, which proves that the extractive is I-type collagen.Research on physicochemical property.taking pH, collagen concentration and NaCl concentration as the conditional variables, analysis and research towards the collagen solubility, foamability, foaming stability, emulsibility and emulsion stability is carried out,The optimal conditional combination for solubility is that temperature is 40℃, pH=9 and NaCl concentration is 0.6 mol/L. The optimal conditional combination for foamability is that pH=8, collagen concentration is 0.2mol/L and NaCl concentration is 0.4mol/L. The optimal conditional combination for foaming stability is that pH=6, collagen concentration is 0.2mol/L and NaCl concentration is 0.2mol/L. The optimal conditional combination for emulsibility is that pH=10, collagen concentration is 0.6mol/L and NaCl concentration is 0.4mol/L. The optimal conditional combination for emulsion stability is that pH=8, collagen concentration is 0.2mol/L and NaCl concentration is 0.4mol/L.After carrying out a comprehensive analysis towards each factor, it obtains that When the pH value was reached 8, the NaCl concentration is controlled to 0.4mol/L, and the temperature is 30℃～40℃, the I-type collagen extracted from bovine bone is optimal in above-mentioned physicochemical property.