Design, Synthesis And Properties Of A Novel Fluorescence Probe For Copper (â…¡) Ions Based On Quinoline

Fluorescence molecular probes with high sensitivity and good selectivity have attracted considerable attention because of the probes’important application in the biological imaging and detecting different metal ions in recent years. Fluorospectrophotomery is a high sensitive method of determining metal ions compared with other analytical methods. Therefore, to design and synthesize many novel fluorescent molecular probes have been one of research focuses in the field of chemistry.Copper is an essential metal element in life organism. However, copper overload in humans causes various neurodegenerative diseases. Excessive copper in the environment will cause environmental pollution. Many detection methods, for example atomic absorption spectrometry and inductively coupled plasma mass spectrometry (ICP-MS), have been used for Cu2+ detection. However, those methods are both complicated and costly compared with fluorescence determination.A new photoinduced electron transfer (PET) fluorescent molecular probe for Copper (Ⅱ) ions has been designed and synthesized in this paper. Utilizing the 8-Hydroxy-2-methyl quinoline and Ethyl 2-chloroethoxyacetate, after three synthetic steps, Di-(2-Picolyl) Amine participated in the reaction in the presence of NaB(OAc)3H, then a new fluorescent molecular probe S1 has been synthesized.In this paper, all of the synthesized compounds have been characterized by ’HNMR. Fluorescence spectrometers and ultraviolet spectrophotometers have been used to detect the ultraviolet and fluorescence properties of probe S1. The probe S1 behaves a remarkably quenching phenomenon especially in CH3CN/H2O solutions (v/v,4:1, pH 7.68) after the addition of Cu2+. The fluorescent response of S1 to Cu2+ is hardly affected by other metal ions with good selectivity and high sensitivity. The fluorescence titration experiment indicates stoichiometric ratio of S1 and Cu2+ is 2:1. Therefore, fluorescence probe S1 can be applied to recognize and detect Cu2+.