| Chemiluminescence analysis study is focused on finding a new chemiluminescence reaction system to expand its scope of application. Because of the unique properties, nanoparticles have been favorablely adopted to design novel analytical method in the chemiluminescence. This thesis consists of two parts. The first part is the review, which described the preparation of noble metal nanoparticles, their physical and chemical properties and appliaction, focusing on chemiluminescence analysis of the noble metal nanoparticles. The second part is as follows:I:Silver nanoparticles-initiated chemiluminescence reaction of luminol-AgNO3 and its analytical applicationAg+ has been considered as an inernt chemiluminescent oxidant. In this work, we found that Ag+ could react with luminol to produce strong chemiluminescence (CL) in the presence of silver nanoparticles (AgNPs).The AgNPs with smaller size could initiate the stronger CL emission.To investigate the CL mechanism of AgNPs-luminol-AgNO3 system, the UV-visible spectra and the CL spectrum of the CL system were obtained. On this basis, the CL reaction mechanism involving catalysis was proposed. Compared with the reported nanoparticles-luminol-H2O2 CL system, the AgNPs-luminol-AgNO3 CL system has the advantages of low background and good stability. Moreover, we constructed a new chemiluminescence immunoassay for human IgG. Under optimized conditions, the CL signal intensity was linear with human IgG concentration in the range of 10-100 ng/mL, with the correlation coefficient of 0.9905.The detection limit is 3 ng/mL.II:Chemiluminescence determination of Mercury (Ⅱ) using Luminol-H202-AuNPs SystemWe describe simple and sensitve chemiluminescence detection Hg2+ using the interaction of ss-DNA and Au nanoparticles, the specific binding of Hg2+ and the thymine base, the difference catalysis activity of dispersed AuNPs and aggregated AuNPs on luminol-H2O2 reaction system. While in the abscence of Hg+ ions, T-only single strand oligonucleotides could adsorbed on AuNPs, which stabilized in a high salt concentration, producing weak CL emission. While in the prescence of Hg2+ ions, the two T-only single strand oligonucleotides could form the T-Hg2+-T coordination, which made AuNPs less protected then aggregated at the same salt concentration, leading strong CL emission. Based on this result, the detection of Hg2+ can be indicated by the difference of CL singal of luminol-H2O2 system. By changing thymine bases in DNA oligonucleotides, the detection range could be turned. When we used seven thymine bases in DAN sequence, a linear detection range from 1.0×10-8-1.0×10-6 mol/L can be obtained. By adding thymine to ten bases and thirteen bases, the detection ranges of the two sensing systems are obviously different from the seven ones, which are 1.0×10-9 mol/L-1.0×10-7 mol/L and 1.0×10-10 mol/L-5.0×10-9mol/L. Besides, in the presence of EDTA (as masking agent), the assay shows excellent selectivity for Hg2+ ions over other metals including (K+, Co2+, Ca2+, Cr3+, Fe3+, Mg2+, Al3+, Cu2+, Pb2+, Mn2+, Zn2+and Cd2+). Experimental result shows T-only short ss-DNA can detect higher concentration of mercury ion, while the longer ones can be used to detect relatively low concentration of mercury ion. |
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