4 Alaudidae 12srrna Genes Pcr Amplification

For forest public security case involving wild animals in the process, inevitable meeting of the wild animals, and their appraisal of the forest public security, in order to explore a limited conditions for forest public security handling and convenient method in the identification, experiment and research.12SrRNA mitochondrial genome sequence, because of its highly conserved and species-specific may be used for species identification of more and more. In this study,12SrRNA sequence Braun species in Inner Mongolia in the five kinds of (Melanocorypha mongolica, Calandrella cinerea, Eremophila alpestris, Alauda arvensis, Galerida cristata) for species identification, which angle Braun 12SrRNA sequence is known, Asian Short-toed Lark is not collected without the experiment. The test uses five samples (one sample as to be determined) plus liquid nitrogen, grinding the muscle extract genomic DNA, using bird-specific fragment was amplified 12SrRNA part of the universal primers on genomic DNA amplification, the amplified products obtained sequencing, which identify the type of bird species to be determined, to identify unknown birds methods.