.532 Nm, A Weak Laser Irradiation Of Rabbit Red Blood Cell Hemolysis Effect

Bio-effects of low intensity monochromatic light radiation are interdisciplinary research fields, which is of important prospect and wide attention.The hemolysis of rabbit's erythrocyte in vitro under irradiation of 532nm semiconductor laser is investigated. The results show that the hemolytic rate of 532 nm semiconductor laser is signifintly lower than 660 nm semiconductor laser, and the hemolysis is not positive correlation with laser power.The rabbit's fresh erythrocyte suspension washed by physiologil saline is the object to be observed, sodium heparin solution is the anticoagulant. The ratio of erythrocyte hemolytic rate of irradiation group and control group will be investigated.The hemolytic rate induced by 532nm green LED light, 623.5 nm red LED light and that by532nm green laser is studied in this paper. The results obtained show that there is no signifint difference between effects of 532nm LED and 532nm laser. The hemolytic rate of 623.5 nm LED radiation is signifintly higher than that of 532 nm LED light and 532nm laser. The hemolysis-effect of 623.5 nm LED is similar with that of 660nm laser. The effect is time-delayed (There was no signifint difference between the test and control groups when tested in two hours, but after 24 hours the hemolysis of control group is prominent). Hemolysis is positive correlation with laser power.For erythrocyte, green light absorption is much higher than red light absorption. But the results of experiments show that the hemolysis induced by green light is much lower than that induced by red light. That means the absorption does not play a major role in hemolytic effect of erythrocyte induced by light irradiation.This research may offer new experimental support for the study of bio-effects of low intensity laser radiation and its mechanism. Then impact factor and biologil signifince are analyzed. The results of this paper will provide important experimental support and theoretil guidance for the study of mechanism about photo-blood interaction, low-intensity laser biologil effect and low-intensity laser therapy. The research method of this paper may provide a new way to study the low-intensity laser biologil effect.