Spirulina High Cell Density Culture Device Design And Culture Conditions Optimized

An air-lift plate photo bioreactor was designed to cultivate the S. platensis, based on the principle of photo bioreactor design after comparing the main categories and development situation with several photo bioreactors.Then introducing a few optimization methodies used in optimization medium and technological conditions, finally determining to select response surface methodology to optimize the cultivation condition of S. platensis according to the feature of Response surface methodology firstly.Emplying the response surface methodology to optimize the cultivation condition of photoautotrophy and nutritional ingredient of mixtrophy, relatively.The whole experiments were accomplished in the self-made 10.0L air-lift plate photo bioreactor. In the photoautotrophy , four critical factors selected for investigation were light intensity, air flow, time of cultivation and volume of medium, research the optimization levels and the relations between these factors and building a quadratic regression equation with DW as the dependent:DW= 1.25+0.096A+4.792E-003B+0.039C-9.792 E-003D+3.125 E-004AB-0.018AC+1.688 E-003AD+6.250 E-005BC+5.625 E-004BD+4.375 E-004CD-0.1A~2-0.057B~2-0.024C~2-0.033D~2by solving the inverse matrix from the equation, the optimal conditions for obtaining the maximum 1.277g/L of DW were 4.4klx, 212.2L/h,8.8d and 7.2 L respectively. The experiments for validating the quadratic regression equation proofed the the model had played well in a role in the practice..On the base of the photoautotrophy, the first step of mixtrophy was one-factor Experiment to search the nutritional ingredient which could promote the growth of SP obviously. After that Factional Factorial Design was carried out to look for these factors that had significant influence to the growth of S. platensis. Subsequently, Response surface methodology was used to optimize the concentration levels of significant nutritional ingredient, the quadratic regression equation with D W as the dependent was:DW= 1.66-0.067 A-0.04B-0.011C+8.250E-003AB-8.500E-003AC -1.750E-003BC-0.066A~2-0.042B~2-7.909E-003C~2by solving the inverse matrix from the equation, the optimal levels for obtaining the maximum 1.688g/L of DW. The experiments for validating the quadratic regression equation proofed the the model had played well in a role in the practice. Eventually the result of experiment identificated the interval of 48 hours with adding the nutritional ingredient was the optimal, the maximum Dry weight was 1.822g/L, it is more than 3 times than the cosmically produce. The result of experiment proved that the bioreactor is propitious to cultivate the S. platensis.