.hplc / Ms / Ms Technique In The Study Of The Origin Of Life

Life science has been recognized as one of the most frontiers of the 21st century. With the completion of the mapping of the human genome, life science is progressing into the proteomics age. Similar to the first gene mapping which was completed ahead of schedule using a capillary electrophoresis sequence technique, proteomics research will also develop quickly because of the introduction of the modern bio-mass spectrometry (bio-MS) technique. Together with modern separation techniques, the application scopes of the bio-MS technique will be extended, and high performance liquid chromatography/mass spectrometry (HPLC/MS) coupling technique is becoming a necessary support technique for life science research.In the chapter 2 and 3 ,using electrospray ionization mass spectrometry (ESI/MS), HPLC/MS and other analytical techniques,we have established a HPLC/ESI/MS methodology for simultaneously determining trace mononucleoside momophosphate, dinucleoside monophosphate and dinucleoside diphosphate in pyridine reaction between four oldest N- (O, O-diisopropyl) phosphoryl ammo acids and the mixed nucleotides (A+C+G+U). In this system, mononucleoside momophosphate (XMP),dinucleoside monophosphate (XpY) and dinucleoside diphosphate (XpYp-cyclo)(X,Y=A,C,G,U) were observed, the possible target products were validated by analyzing their cleavage pathways and their corresponding specific ions. At the same time, HPLC-ESI-MS/MS was used to identify the sequences of dinucleotides. Backbone fragmentation was characterized by abundant c-ions as the major sequence-defining fragment ion series.The results demonstrated that the four model reactions produced different kinds of XMP , XpY and XpYp-cyclo at various range of contents, which was attributed to the different reaction activity and selectivity. This HPLC/ESI/MS technique could be potentially used to further investigate similar reactions between Dipp-aa and the mixed nucleosides. It might provide experimental proof for the assumption of Dipp-aa being the smallest intermediate of co-origin of nucleic acids and proteins during origins of life.AbstractIn the chapter 4, the purity of lansoprazole in the medicine and the structure of subsidiary product were determined by RP-HPLC-ESI-MS/MS.The result...