Study Of Higher Plants Nutritional Effects Of Amino Acid Nitrogen

This study was proposed to investigate the effects of different amino acid nitrogen on crops under sterile sand culture. It was carried out that Chinese cabbage, green gram, rice and wheat were cultured with glycine, glutamate, lysine or ammonium sulfate as a sole nitrogen source under sterile sand culture conditions. The effects of glycine and ammonium sulfate mixture on wheat, Chinese cabbage and mung bean were also carried out using 15 technology. Arabidiposis grown with glycine as sole nitrogen under sterile sand culture was also carried out to investigate whether plants could finish its whole life cycle only supplied organic nitrogen. The results obtained were followed. 1. Study on sterilization method to seed for plant organic nutrition experiments Obtaining sterile seedlings from seeds is an important step for plant organic nutrition experiments. The plant seeds of Chinese cabbage, Pai-tsai, green gram, 94 rice and wheat were sterilized with different combinations of three sterilizing solutions (70% ethanol, 0.1%HgC12 and 2%NaC1O or 10%H202) in these experiments. The results showed that the effectiveness of combinative sterilization to plant seed was affected by plant species and seed storage time. The combinative sterilization procedure recommended was the following. (1) Seeds were firstly immersed in ethanol (70% VIV) for 1 mm and then rinsed three times with sterile distilled water. (2) Secondly immersed in sodium hypochlorite solution (2% NaC1O active constituents) for 5 mm and then rinsed five times with sterile distilled water. (3) Finally immersed in mercuric chloride (0.1% HgCl2 W/V) for 5mm and then rinsed with sterile distilled water six times. If the plant seeds were very sensitive to 2% NaClO, it would be advisable to use 10% H202 (v/v) instead of 2% NaClO. 2. The effects of different nitrogen forms on rice seedlings under sterilized culture at high temperature The effect of different N sources (ammonium sulfate, glutamate, glycine and lysine) on rice seedlings grown in high temperature (about 370C during lighting period and about 33 0C during dark) sterile sand culture was studied. The dry matter of plants grown 20 days with ammonium as sole N source was significantly lower than that grown with amino acids, and also slightly lower than that grown free N (control), indicating ammonium toxicity. The total N of plant cultured with amino acids was similar to that with ammonium, and was 50% higher than control. Compared with the control, GOT or GPT activity of roots or leaves of plants grown with ammonium (10 or 500 mg N/L) increased twice, perhaps relative to ammonium toxicity. Grown for 15 days in low N concentration (10 mg NIL), GOT activity in leaves of plants with glutamate as sole N source and GPT activity in roots and leaves and GOT activity in roots of plants fed with glycine significantly increased, and GOT activity in leaves of lysine-fed plant decreased. Treated with 95 high N concentration (500 mg NIL) for 6 h, the activity of GPT was 5.7, 9.4, and 1.9 times control in roots, respectively, for glycine-, glutamate- and lysine-treated plants; and was 1.8, 2.3 and 1.4 times control in leaves, respectively. 3. The effects of different nitrogen forms on wheat seedlings under sterilized sand culture The d...