Mice At Different Developmental Stages Of Nos, No, Et And Cgrp Expression In The Spinal Cord / Brain

In recent years, with the study development of the research on central nervous system, the relationship of some new-found neurotransmitters and live polypeptides in the central nervous system with the diseases that the aged often suffer from has been paid more attention to. The research on these material has already become the front of the develop neurology. Some researches show that nitric oxide , endothelin and the Calcitonin gene-related peptide have intimate relationship with central nervous systems, and their content changes obviously in the diseases that the aged often suffered from. This text tries investigating the relationship between the contents of the three substances in central nervous systems and age, in order to study their function.This experiment selected healthy female mice of the species in Kunming, which were divided into three age segments: 15-20 d, 90-95 d, about 270d. The content of nitrogen synthase(NOS) , nitrosyl (NO₂^-), endothelin (ET) and Calcitonin gene-related peptide (CGRP) in central nervous systems of every age group was measured by NADPH-diphorase histochemical staining , the Griess method and radioimmunological analysis.1. It was found that the contents of NOS in every segment of spinal cord descended with the development of mouse, especially low in the aged mouse's spinal cords. The result shows that the composition of NO was changing throughout the development, and it is signification to research Ihe expression of NO in the central nervous system.2. The experiment of NO₂^- showed, the concentrations of NO₂^- within the cerebrum tissue of mouse were rise in the upgrowth and descend in the aging. It was speculated that the NO took an important place in the development of cerebrum.3. The radioimmunological analysis showed: ①The concentrations of ET do not change obviously with the age, some articles mentioned that ET and NOkeep homeostasis in blood in the course of nature, peobably due to the different concentrations in different age group. ?The CGRP content has inverse correlation with the development of mouse, and the range of undulation was different in different age groups. It can be concluded that CGRP contents has some relationship with age and it might influence the development of brain.The results above are helpful to clarify the connection between the contents of NO, ET, CGRP and the development stage of mammal central nervous system, offer some information about the function of them in the development of mammal central nervous system, and provide a thoughtway for the research of anti-senescence.