Digital Modification And Testing Technology, Cell Microinjection

With the development of biotechnology, the development of the technology of cell microinjection has been achieved quite greatly in the area of cell manipulation. There are so many researches at home and abroad, while the micro-fluidic digital technology studied in the micro-system lab in Nanjing University of Science and Technology lately has been developed commendably. There are some creative theories and basic concepts in the micro-fluidic digital technology. The micro-fluidic digital technology is uttermost important for the research of the micro-fluid system and the process of the micro-injection technology, and the micro-fluidic digital technology will be applied widely in the area of cell micro-injection.In this thesis, the content based on the micro-fluidic digital technology above the applied research of cell micro-injection is systematically designed. On the base of the brief description of the drive and control system of micro-fluid and the experimental setting of the technology of micro-injection, a novel process of the hydrophobe treating used to mend the characteristic of the inner and outer surface on micro-pipette is designed, which can improve greatly the injected effect and eliminate the disadvantage such as seepage or regurgitation during injection. The author puts forward the way of measuring the inside diameter of the micropipette. The program of measuring micro injection amount is designed. The original data of the micro injection experiment are explained and interpreted at last in the thesis.