Spider Dragline Silk Protein Gene Cloning And Partial Sequencing

Spidroin(spider silk protein) is a kind of very special fibrin. The beta-sheet, alpha-helical and its second structures make it have strong strength, high tenacity and elastic extensibility. Spidroin is superior to other protein fibers. These properties make it useful in medicine, especially, in tissue engineering. Its sequences and structures have been studied clearly. Partial genes of the dragline have been cloned successfully. But the expressed protein is different from the known protein sequences. Because of the repetitive motifs, unsteadiness and long sequences, the cloning of the whole genes is very difficult. For its properties, Spidroin has good prospect of application in many fields.In this paper , we constructed the genomic DNA library of Nephila clavipes with the vector SuperCos 1 Cosmid. Then, we find out the write clones through the chemis hib. During the working , three probes which we used are from the published sequences of spidroin. after this,a genie library of subclone of dragline was constructed. Through determining clones' sequence we obtained some sequences of spidroin.