| Mao-Tai wine produced in Guizhou province China is famous all over the world. Its unique taste has decided its important position as the national wine. There is a close relationship between the fermentation process of Mao-Tai wine, the nature environment of Maotai town and its special flavor. Therefore, there is a significant importance to study the diversity of microbial environment of Chishui River that passing through Maotai town.As the most abundant and largest group in nature environment, microorganisms play an indispensable role in the survival and development of human beings. Therefore, classification for those microorganisms has a positive effect on understanding the world completely. Our country has a rich resource, vast region, thus the microbial resource is extremely rich. Therefore, there needs to be a continuous job to complete the classification process. All in all, classification, identification and naming have become the three parts which constitute the microorganisms’classification process.Flavobacterium was first proposed by professor Bergey in1923, and strictly described in1996by Bernardet. Flavobacterium Cells are usually rod-shaped, margin is smooth or irregular, rounded or slightly tapered, has no gliding motion, no flagella, and are gram negative bacteria. Typically speaking, Flavobacterium contains flexirubin pigment or carotenoid, but there are also exceptions.Two novel strains, T9T and T10, were isolated from water samples collected from Chishui River flowing through Maotai town, Guizhou, southwest of China. The isolates were yellow-pigmented, Gram-reaction-negative, rod-shaped, non-motile, and aerobic. Phylogenetic analysis based on16S rRNA gene sequences showed that the isolates belonged to the genus Flavobacterium, and shared less than97%sequence similarity with recognized Flavobacterium species. The novel species were able to grow at20-37℃(optimum25℃), pH7.0-10.0(optimum pH7.0-8.0) and in0-0.5%(w/v) NaCl concentrations (optimum0.5%). The predominant fatty acids were iso-C15:0, iso-C15:1G, anteiso-C15:0, iso-C15:03-OH and iso-C17:03-OH, and menaquinone-6(MK-6) was the main respiratory quinone. The major polar lipids were phosphatidylethanolamine (PE) and phosphatidylmethyrlethanolamine (PME). The DNA G+C contents of T9T and T10were37.7mol%and36.4mol%, respectively. According to the phenotypic and genetic data, the strain T9T and T10represent a novel species in the genus Flavobacterium, for which the name Flavobacterium maotaiensis sp. nov. is proposed. The type strain is T9T (=CGMCC1.127121=JCM xxxx).Another novel strain, T3T, was also isolated from water samples collected from Chishui River flowing through Renhuai, Guizhou. The isolates were Gram-reaction-negative, rod-shaped, non-motile, yellow-pigmented and aerobic. According to the phylogenetic analysis based on16S rRNA gene sequences, the isolates belonged to the genus Flavobacterium, and shared less than97%sequence similarity with recognized Flavobacterium species. The novel species were able to grow at20-34℃(optimum28℃), pH6.0-11.0(optimum pH9.0) and in0-2%(w/v) NaCl concentrations (optimum0%). The predominant fatty acids were iso-C15:0, C16:00,iso-C17:03-OH and iso-C15:03-OH. Menaquinone-6(MK-6) was the main respiratory quinone, and the major polar lipids were phosphatidylethanolamine (PE). The DNA G+C content of T3T was44.1mol%. Based on the phenotypic and genetic data, the strain T3T represents a novel species in the genus Flavobacterium, for which the name Flavobacterium renhuaiensis sp. nov. is proposed. The type strain is T3T (=CGMCC1.129267=JCM xxxx).In this research, we have studied three strains which contain two different species of the Chishui River, Maotai Town. We did the taxonomic research and comprehensively analyzed the characteristics of these three strains. Furthermore, we have studied completely about the Flavobacterium species and also, new species has been proposed and added to the data. All in all, a great contribution has been made on the microorganism classification area. |
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