| In this study, defatted coconut seed coat as experimental materials, analysis of seed coat polyphenols coconut skim the best extraction process, using high performance liquid chromatography of skim coconut seed coat extract polyphenol and polyphenol content of the components, and a preliminary evaluation of defatted coconut seed coat polyphenols antioxidant activity. Phosphate buffer and then use the seed coat extract of defatted coconut protein, the functional properties were studied, prepared by the appropriate choice of protease digestion peptides, the biological activity of peptides analysis. For skim coconut seed coat application, the use of organic solvents to remove anti-nutritional factors which, added after drying for Chicken in Chicken feed feeding experiments to study the coconut seed coat skim the growth of multi-Chicken to to skim coconut seed coat development and utilization provide a theoretical basis. Specific research contents and results are as follows:(1)Single factor experiments, initially identified skim coconut seed coat polyphenols extracted the optimum conditions, IE extraction solvent isopropanol, solid-liquid ratio1:5, extraction time2h, extraction temperature60℃. Phenol by colorimetric method to determine the forint total phenolic content, determined by the optimization of the extraction rate of polyphenols obtained by7.64mg/g dry matter. Types and the determination of polyphenols using HPLC, the results identified four types of seed coat polyphenols coconut fat composition, IE p-coumaric acid; chlorogenic acid; rutin; kaempferol.(2)To skim coconut seed coat methanol extracts of polyphenols as experimental materials to BHT as a control to study the reduction of Fe3+and the ability to inhibit the oxidation of edible oil. Experimental results show that fat coconut seed coat methanol extracts of polyphenol has significant antioxidant effect, although weaker than the sample antioxidant BHT, but the reduction of Fe3+and the ability to inhibit the oxidation of edible oil, along with the sample concentration increased and increased, and the different extraction solvents showed different antioxidant capacity.(3)The seed coat with coconut skim pH7.8phosphate buffer solution to extract.While its solubility, foaming, emulsifying and other functional properties were determined using pepsin-trypsin digestion in vitro two-step method of skim coconut seed coat protein digestibility was determined by solubility and poor water holding capacity, has good foaming and emulsifying properties, may be used in beverages, ice cream, bread, sausages and other foods. (4)In accordance with skim coconut seed coat protein solution-pre-enzymatic (acid protease)-enzyme inactivation (95℃,10min)-centrifugation (10000rpm,4℃,15min)-vacuum concentration-vacuum drying methods, the coconut seed coat polypeptide. Gallic acid and BHT as a control, research skim coconut seed coat polypeptides on DPPH radical and hydroxyl radical scavenging ability, Fe2+and Fe3+complexation ability of the reduction of capacity. The results show that fat coconut seed coat polypeptide has significant antioxidant activity on DPPH radical scavenging ability and the reduction of Fe3+were lower than gallic acid and BHT; while the hydroxyl radical scavenging ability and Fe+complex is mediated in gallic acid and BHT between. Hydrolysis of amino acid composition and content through the "Practical Handbook of food nutritional analysis" in the method of detection, the results show contains16amino acids, the total content can be achieved13.6g/100g.(5)Will skim coconut seed coat after removal of anti-nutritional factors, the proportion of15%added to the Chicken feed, and there is no fat coconut treated in the same proportion of the seed coat also add, do not add fat to coconut seed coat Chicken Feed as a control to observe the feeding of Chicken with different growth conditions. The results show that it is fat coconut species Pew after the removal of anti-nutritional factors skim coconut seed coat influenced the growth of Chicken. |
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