Study On The Inhibitory Effect Of Paeonol/Magnolol And Its Application In Toothpaste

It is necessary for using toothpaste in people’s daily life as the most directactivities, which could be used for cleaning the tooth, maintaining oral health. Withimproving of people’s living standard, the toothpaste has been changing from ordinarycleaning to functional application.Healthy gums may suffer from plaque induced gingivitis due to various reasons.If not controlled and treated, plaque induced gingivitis will develop periodontitis. It isimportant to suppresses dental plaque and keep tooth healthy. In recent years, moreand more natural herbal extracts were applied to the study of daily chemical products.Among them, Paeonol and honokiol are all reported. But there are not reports on usingthem both. The main research contents of this paper are as follows:The minimum inhibitory concentration tests of paeonol and magnolol onescherichia coli, staphylococcus aureus, candida albicans, streptococcus mutans,fusobacterium nucleatum respectively were performed to check the bacteriostatic effect. Thetest and analysis results of minimal inhibitory concentration in the mixture sampleswith different quality ratio (agar dilution method) showed that both inhibitoryproperties are complementary.The Paeonol and magnolol in accordance with optimal dosage ratio were addedto toothpaste, and the toothpaste was arranged in45℃for3months of destructive test.The appearance, aroma, stability, the change of pH value are used to determine thestability of toothpaste for3years warranty. The high performance liquidchromatography (HPLC) was applied to test the change trend of the effective contentwith different concentration of paeonol and magnolol in toothpaste. The results foundthat the effective content of Paeonol and magnolol in toothpaste decreased graduallywith the time growth. The test of the best formula of1-100dilution degreesbacteriostasis rate of the toothpaste showed that the inhibitory rate reached more than90%.The assessment of toothpaste on plaque status (PI), gingival status (GI) andbleeding on probing (BOP) was carried out by using the method of randomizedclinical trials, every day at least two times in the unsupervised case daily family for3months. Each tooth was recorded with certain examination site, for observing whetherthe toothpaste inhibits dental plaque and reduces gingivitis effect. The resultsshowed that the indexes of gingivitis and plaque of experimental group were lowerthan those in the control group. The toothpaste with addition of paeonol and honokiolcan reduce gingival and inhibit the role of dental plaque.