Determination Of Antibiotics With Gold Nanoparticles By Resonance Rayleigh Scattering Method

As important nanoparticle material, gold nanoparticles (GNPs/AuNP) haveespecial physical and chemical characteristics and have been used in the fields ofimmune analysis, biological probe, biological sensing, drug analysis and metaldetection. In recent years, resonance rayleigh scattering (RRS) technique, with itssimply, high senstivity and rapidly characteristics, has attracted more and moreattention. With the development of RRS, people found that gold nanoparticles couldcombined with biological macromolecules, small organic molecules, such asmedicaments, alkaline dyes and metal ions. Moreover these combination productsexhibit unique RRS spectrum, which made the spectral analysis of GNPs in RRSbecame possible. In this article, we investigated RRS spectral detection of antibioticsby gold nanoparticles.(1) The first chapter introduced the physical and chemical properties of GNPs,the synthhesis methods of GNPs and their application in the biochemical analysis.Moreover introduction of RRS spectra and its applications in various aspects havesummarized. At last, we put forward the idea of this paper.(2) Gold nanoparticles were prepared by sodium citrate reduction procedure. Inacidic medium, the gold nanoparticles could combine with sulfadiazine by the virtueof electrostatic and hydrophobic interaction, developing aggregate with biggerdiameter. The aggregate would arouse RRS intensity enhancing greatly. Effect ofexperimental conditions were investigated, such as the acidity, concentration of goldnanoparticles, the reaction time and the temperature. Under the optimal conditions, agood linearity was obtained between the RRS intensity and the sulfadiazineconcentration in the range of0-80g/L. The detection limits (3σ) was1.65ng/mL.The amounts of sulfadiazine in pill were determined by the proposed method and therecovery was99.3%-101.0%.(3)Gold nanoparticles in size of6.5nm were composited by a NaBH4-trisodiumcitrate procedure. The aptamer of Kanamycin was used to modify the goldnanoparticles to form a stable aptamer-GNPs complex that would not aggregation byNaCl. After adding kanamycin in the solution, it could affinity interact with theaptamer to form a more stable composite while GNPs were released. The uncombinedGNPs aggregated to large clusters, which could cause the increase of RRS intensity in high concentration of NaCl. Under the optimal conditions, a good linearity wasobtained between the increased RRS intensity and the kanamycin concentration in therange of0.02-0.3mg/L, with a detection limit(3σ) of2.3μg/L.(4)Gold nanoparticles were prepared by Sodium borohydride reductionprocedure. In high concentration of ions, GNPs would aggregated to large clustersthat led solution color change from red to blue. But when adding cefazolin to thesystem, the aggregation of gold nanoparticles would be suppressed, and the color ofsolution remains red. Then the amounts of cefazolin could be detected according tothe increase of RRS intensity. Under the optimal conditions, the increased RRSintensity was linear over cefazolin concentration in the range of0.5-5.0mol/L, with adetection limit(3σ) of0.03mol/L.