Study Of Biological Sensing Electrochemical Analysis Based On Photosensitivity Of Plant

Cryptochromes (cryptochromes, CRY) are blue light receptors that regulate circadian clock in animals, plants, bacteria and other living organisms. The related research on CRY is increasing, and mainly adopts spectroscopy method to study the electron transfer process after the stimulus. In this work, the electrochemical analysis method was applied to study the photosensitive effect of plants, and find out the ways to build photosensitive biological sensing model research, the main content is divided into three parts:First of all, CRYs are flavoproteins which structure is similar to glucose oxidase. Since glucose oxidase has been widely investigated in electrochemical analysis, it is a better method to verify the reliability of the sensor channel to build glucose biosensor. Modified electrodes were characterized by cold field emission scanning electron microscope (SEM), AC impedance spectroscopy (EIS), X-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV), open circuit potential time curve (OCPT) and current time curve (I-t), respectively. We obtained a simple and novel method of establishing glucose sensor.Second, adopted blue light receptors cryptochrome1(CRY1) as the research object, according to the path of the glucose sensor and made appropriate adjustments, three methods were used to modify CRY1on the surface of electrodes, the electron transfer process was characterized through electrochemical analysis, explore the changes of charge and structure of cryptochrome under light. However, there is no obvious electrochemical analysis results obtained from experiment which are restricted by cryptochrome’s concentration and other reasons.Third, considering the effect of protein’s activity and regulation pathways are complicated, and the whole extracts from plant leaves may meet all the requirements. Adopting the whole protein extract from plant leaves as the research object, I-t curve was applied to observe the current difference of protein extract before and after the light stimulus. The results showed that the current increased with the concentration of extract, illumination time and the light intensity stimulated by light. After a semipermeable membrane dialysis extract, the I-t test showed that the extract of small molecular compound had a unique electrical signal response to light, especially for blue-violet light, and the response is closely associated with the plant growth cycle.