Isolation And Purification Of Polysaccharides

Jujube is a deciduous shrub or small tree plants jujube, jujube is also ripe fruit.Flowers smaller than honey, is a nectar plants. Originating in China, has grown in most parts of China, is mainly produced in Shan xi, Shaaban, Hebe, Shandong, Helena,Gans Mince and several other provinces and Sinkiang jujube traditional production emerging field. Autosexing’s geographical location and climate are very special, differentfrom the mainland coast. Here the dry climate, long hours of sunshine, the temperature difference between day and night is obvious. This industrial development in Sinkiang jujube creating a better field of development, and out of Sinkiang, the indicators ofthe real estate and sensory quality jujube quality, nutrition and other inland provincesare better than dates.Sinkiang jujube future advantages can be called the country’s largest production ba sedate, and how to day’s most important job Bisto Devol ethe counterscientific technology,industrialization,standardization,specialization.This is the great challenge we will face in Sinkiang.To date from the20th century to be used as food and medicine,to share regular consumption,mainly due to backoff gas,spleen and stomach,body tired fatigue, eat less, loose stools,blood deficiency chlorosis,hysteria treatmentof women and other widely used.After years of pharmacological studies have found, jujube contains variety of biologically active substances, such as jujube polysaccharides,flavonoids,saponins,terpenoid, alkaloids,cyclic adenosine metaphosphate(AMP),cyclic guanosine metaphosphate(GMP), etc.there area variety of treatment impact on human health.Is the traditional medicine of the use of fresh fruit.However, the extent of jujube relatively lore source development and utilization of the lack of systematic research on its corresponding processing technology and functionality.Due date polysaccharide withQi tonic,nourishing the nerves,such as the stomach,spleen and stomach, lack of blood,fatigue, weakness, insomnia and other functions,has aroused concerns of consumers.And won the interest of researchers.This paper systematically studied the most commonly used plant polysaccharides extraction, separation and purification methods, and jujubepolysaccharide antioxidant analyzed.In this experiment,the polysaccharide yield and pure as the markerextractionstudiesjujubepolysaccharidepolysaccharidelasthot water bath extraction method,extraction method proposed four ultrasonic extraction, microwaveextractionandenzymaticextractionmethodtocomplexpolysaccharideswere compared to identify not only the cost, but also improves resource utilization and high yield method.Using response surface analysis, to explore the complex enzymatic extraction process optimization Adsuki Jun azo polysaccharides.By pre-dates the experiment to determine the effect of biologicalfactorspolysaccharidecomplexenzymaticextractionyield and purity are hydrolysis temperature,hydrolysis time, hydrolysis PH,solid-liquid ratio.Finally, response surface optimization experiments to determine the optimum extraction conditions for polysaccharides:are action temperature of56~65℃,hydrolysis time50~60min, PH5.6~6.3,enzyme volume1%.With conventional hot water bath extraction and ethanol precipitation method compared to the composite enzymatic method greatly reduces the extraction time on the one hand,on the other hand polysaccharides yield from8.19%to12.33%.By macro porous resin and polyamide discoloration was found, where the recovery polyamide bleaching effect and polysaccharides are high. Polyamide bleaching bleaching effect is not only good but also to proteins and some magazines. Thus, this experiment determined as the polyamide bleaching agent efficiency is desirable. Studies todetermine the amount of polyamide is four times the desired decoloration of the polysaccharide sample date, the best conditions eluted with deionized water, the column was twice the volume of water in the polyamide column experiments, the elution rate was1.5mL/min.The experimental results showed that the antioxidant activity, polysaccharide test has good antioxidant capacity, and in the range of test concentration, total antioxidant ability ofpolysaccharide, hydroxyl radical and1,1-two--2-three dinitrophenylhydrazine free radicalscavenging rate was linear. The sample polysaccharide on various forms of active oxygenscavenging effect and all, clearance rate and concentration.Through the above experimental results, the separation from defective or processing by products Akesu Jun jujube activity of purified polysaccharide preparation jujubepolysaccharide oral solution, thereby improving the utilization of early resources.