The Study On Antioxidant Effect Of Different Kinds Of Mango In Vitro Simulated Gastrointestinal Tract Model

In this paper, mango was used as raw materials to study the changes of polyphenol,flavonoids content and antioxidative activity (ORAC) in different stages of gastrointestinal digestion in vitro, and investigated the role of the digestive chemical environment (gastric acid environment and intestinal alkaline environment)and digestive enzyme (pepsin, pancreatin and the bile) played in the digestive process. Compared in vitro digestion with chemical extraction, in vitro digestion reflects the metabolism of mango in the human body more true, provides scientific basis for rational evaluation of mango antioxidant function. Main conclusions of this study are as follows:(1)After in vitro simulated gastrointestinal digestion, mango polyphenol will get a lot of release, during gastric digestion phase, Guireqiaho, Tainong,Jinhuang mango polyphenol released by2.7,3.4,5.7fold of initial amount, respectively. Flavonoids released by4.62,1.56,1.64fold of initial amount, respectively. During intestinal digestion phase, the release amount of polyphenols were1.08,1.52,1.60fold of intestinalO, the release amount of flavonoids were1.68,2.18,1.97fold of intestinal0.(2)After the in vitro digestion, antioxidant activities of Mango (ORAC) will increase, during gastric digestion phase, Guireqihao, Tainong, Jinhuang mango antioxidant activity (ORAC) were4.59,3.46,13.46fold of initial amount. Antioxidant activity of intestinal digestion phase increased by1.65,1.78,1.54fold of intestinal0, respectively.(3)Comparing the three kinds of mango antioxidant activity in the digestivephase of polyphenols, values obtained, GUI heat No. seven, Taiwan, therelease of Jin Huang mango polyphenol and antioxidant activity ability as tGuireqihao> Tainong> Jinhuang mango.(4)Compared three kinds of mango in the digestion of each stage group (blank group, acid group, sample group) the variation betweenpolyphenols, antioxidant activity of polyphenols and chemical environment, the gastrointestinal digestion of three mango did not affect the release of digestive enzymes, and it plays a very important role, there is a closecorrelation between polyphenol content and antioxidant activity of mango.(5)Under the following conditions, mango digestive fluid can be well separatedin liquid chromatograph, conditions:The mobile phase.A.0.2%aqueous solution of acetic acid,B.acetonitrile,Detection wavelength.258nm,Chromatographic column4.6x250,5um AgilentThe elution gradient:0-20min, the concentration of B increased from5%to10%;20-25min, B decreased from10%to5%; the flow rate was0.5mL/min,the column temperature of40℃.