Preparation Of Novel Noble Metal Nanomaterial And Its Application In Electrochemiluminescence Immunosensor

Simple, sensitive and rapid detection of tumor biomarkers plays an important in earlydiagnosis and screening, monitoring disease recurrence, and therapeutic treatment efficacy toimprove long-term survival of tumor patients. Many analytical techniques such aschemiluminescence assay, enzyme-linked immunosorbent assay, radioimmunoassay andelectrochemical assay have been widely employed. Among these methods, electrochemistyhas been widely used in food, medical and environmental fields due to its high sensitivity, fastresponse and simple operation and so on. For resource-poor regions and developing countries,fabrication of low-cost, portable and high-sensitivity biosensors is a difficult task.In this paper, in brief, the proposed biosensors combine microfluidics paper device withthe applications of electrochemistry. Then, a novel biosensor platform based on noble Agnanomaterials modified paper working electrode was constructed, which has superior electricconductivity and large surface area and serve as an effective matrix for biomoleculesimmobilization. Finally, several porous noble nanocomposites with excellent biocompatibilitywere using as signal labels. Using dual-signal amplification, we developed biosensors forrealizing the point of care testing, the main works would be described as follows:1. We designed a3D paper-based electrochemiluminescence (ECL) multiplex origamiimmunodevice. Ag nanoparticles modified paper working electrode (Ag-PWE) was chosen assensor platform for immobilizing biomolecules with good bioactivity and catalytic activity. Atthe same time, glucose oxidase functionalized nanoporous gold-carbon spheres was used as asignal amplification label. The proposed immunodevice was investigated for detection ofthree cancer markers.2. An ECL regent named as CdTe quantum dots (QDs) with good photostability and lowcost and3D nanoporous silver (NPS) were prepared. The prepared CdTe QDs functionalizedNPS was designed as an ECL label. The newly developed Ag-PWE was once more used asgood biosensor platform. An ECL paper-based device was developed for highly sensitive andspecific detection of carcinoembryonic antigen (CEA).3. Based on the single-analyte detection, a multi-analyte electrochemical (EC)immunodevice was designed and fabricated to detect two cancer markers. The novel3D nanoporous silver modified paper working electrode (NPS-PWE) was developed for the firsttime with superior conductivity and good biocompatibility. On the other hand, chitosan waslargely used as a chelating agent for the absorption of metal ions. We successfully fabricatednanoporous gold-chitosan (NGC) for fabrication of versatile NGC-metal ion (Cu2+and Pb2+)tracers. A highly sensitive EC immunosensor was developed based on NPS-PWE andNGC-metal ions.4. Due to superior performance of3D Ag nanomaterials, we also successfully fabricated3D cuboid silver modified origami paper device (CS-PWE) as new sensor platform. And3Dnanoporous silver-chitosan hybrids were fabricated as a carrier to adsorb (Cu2+and Ag+)metal ions. Using dual-signal amplification strategy, a dual-analyte EC immunodevice wasdeveloped for detection of cancer markers.