The Process Of PAHs Solution Degrading Bacteria Immobilized Rot

Aspects of immobilized microbial inoculum, repair of organic contaminated soilimmobilization carrier screening and process optimization, made a lot of progress inresearch in recent years, but for the immobilized microorganism adsorption to theeffectiveness of organic pollutants of biological immobilized microbial inoculumremediation of contaminated soil degradation process of carrier in study on theprocess, and changes in microbial diversity, repair effect is relatively less.Strengthening the research in this field, not only can better understand the mechanismof biological effects of immobilized microorganism repair degradation of soilpolycyclic aromatic hydrocarbons, but also can improve the principle of immobilizedmicroorganism remediation of contaminated soil.Through indoor simulation immobilization carrier decay experiment, theimmobilized carrier of chemical research in decomposition of PAHs residuecomposition, diversity and decomposition of microorganisms in the process, revealedin a fixed carrier rot there PAHs degrading fungi solution under the character andmechanism. Conclusion is as follows:1. With the decomposition, extractable Pyr, B[a]P began to decay after the rapiddecline in the immobilized carrier, and then increased with repeated, to120days andreached the lowest value. The rise of pollutant concentration in the degradationprocess is probably because of the corncob adsorption of Pyr with the decay rate isgreater than the degradation rate caused by the release process.2. From the analysis of the difference of B[a]P content in120days, Pyr, Pyr andcorncob adsorption capacity than B[a]P, therefore, the presence of B[a]P did not affectPyr adsorption; adsorption between Pyr and B[a]P are competitive, so theco-existence of B[a]P content significantly decreased.3. The effects of microbial activity and quantity, the benzene alcohol solublematter, water soluble substances, hemicellulose, cellulose and lignin, the five kinds ofdecomposition products in120days repeated fluctuations.4. Mucor treatment alone is better than the other treatment, especially early. Inaddition, degrading bacteria Pyr, B[a]P are in the indigenous microorganisms, early inthe experiment significantly decreased the content of corncob pyrene, Mucor and soilmicroorganism and adding did not significantly improve the degradation efficiency of PAHs, probably because they have on carbon source, nitrogen source competition.5. Significant positive correlation between the extractable Pyr or B[a]P in thepresence of benzene alcohol soluble matter and water soluble matter content and corncob, showed a significant negative correlation with lignin content. Extractable Pyrmore and water soluble correlated significantly with substance, while B[a]P is morewith benzene alcohol soluble significantly correlated substance.6. According to the analysis of DGGE obtained for each sampling belt, indifferent decomposition will produce new flora of time, to continue to degradepollutants. Soil bacteria and fungi structure added diversity is influenced by pollutantslarger. By the DGGE pattern can be seen, the bright band is likely to be the dominantbacteria in the degradation of PAHs. In addition, from the analysis of diversity indexalso can see the influence of PAHs on evenness of microbial difference is notsignificant.