Effects Of Low-temperature Fermentation Flora Screening And Research

With the continuous progress and development as well as rapid population growth in science and technology, human face to environmental issues and energy crisis is getting worse. Cellulose as a renewable resource on the planet is widespread, and the content is extremely rich. But in our country, more crop stalks and other solid waste is not effectively and fully utilized, processed or burned by a simple method, not only can not make full use of resources and serious pollution of the environment.Heilongjiang province as large grain every year a large number of crop stalks will be left in the field. since the northern autumn and winter low temperature, dry climate, a long period making a large number of crop stalks not timely manner. Agricultural waste is not only rich in cellulose, and contains large amounts of glucose. starch, protein and other available resources Degrading bacteria by screening out the various substances, mixed culture, exploit synergies between strains shorten the fermentation cycle. speeding up the process of composting harmless.In this paper, the rural nature of Daqing straw compost pile and soil as a source of bacteria in culture conditions15℃screening out single strain54. Were screened glucose, starch, protein and cellulose degrading bacteria, get a low glucose degrading bacteria, the minimum growth temperature is10℃; low starch degrading bacteria3, its lowest growth temperature of4℃; low protein-degrading bacteria3. the minimum growth temperature of4℃; cryogenic cellulose degrading bacteria5, the minimum growth temperature is8℃Advantage features low single strains obtained were mixed culture, the influence of antagonistic reaction between strains. and finally got cold mixing a glucose-degrading bacteria cold mixing starch-degrading bacteria4: cold mixed protein-degrading bacteria4; cold mix cellulose degrading bacteria13. Which of the13mixed cellulose degrading bacteria were low total enzyme activity, determination of enzyme activity and endo-exonuclease activity. the combination of the highest total enzyme activity F3F25for0.291IU/g; portfolio F3F8the highest enzyme activity. of1.537IU/g; exonuclease combination F1F8F25highest activity as0.271IU/g. By contrast, the combination of F1F8F25and efficient combination of low F3F8mixed cellulose degrading bacteria.Calculate the low-temperature mixing function degrading bacteria degradation rate, low-glucose degrading bacteria F26at10℃.48h culture degraded rate of48.4%; low starch degrading bacteria group D4F27at4℃,24h degraded culture was27.01%; low protein degrading bacteria group F1F25at4℃,24h cultivation declined7.8%solution; hypothermia group F1F8F25cellulose degrading bacteria at8℃,48h degraded culture was28.08%.Mix on low temperature growth of test functions degrading bacteria were determined and focused research cryogenic mixed cellulose degrading bacteria, grasp the nature of its activity and growth characteristics, for further research, production and mixing functions agents provide theoretical and experimental basis for the handling and use of agricultural solid waste cellulose resource development to provide a way...